World Journal of Microbiology and Biotechnology

, Volume 17, Issue 7, pp 691–696

Production of chitinase and its optimization from a novel isolate Alcaligenes xylosoxydans: potential in antifungal biocontrol

  • R.J. Vaidya
  • I.M. Shah
  • P.R. Vyas
  • H.S. Chhatpar
Article

DOI: 10.1023/A:1012927116756

Cite this article as:
Vaidya, R., Shah, I., Vyas, P. et al. World Journal of Microbiology and Biotechnology (2001) 17: 691. doi:10.1023/A:1012927116756

Abstract

A novel, highly chitinolytic strain of Alcaligenes xylosoxydans was isolated which showed potential for use as an antifungal biocontrol agent for the control of two fungal plant pathogens. It could degrade and utilize dead mycelia of Rhizoctonia bataticola and Fusarium sp. (fungal plant pathogens of Cajanus cajan). In vitro it could inhibit the growth of Fusarium sp. and R. bataticola. Chitin at 10–15 g/l was found to be good carbon and nitrogen source. Alcaligenes xylosoxydans showed optimum chitinase production at 72 h, pH optima at 8 and growth peak at 120 h. Yeast extract, arabinose, Tween 20 and several other surfactants enhanced chitinase production.

Alcaligenes xylosoxydansbiocontrolCajanus cajanchitinase production and optimizationFusarium sp.Rhizoctonia bataticola

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • R.J. Vaidya
    • 1
  • I.M. Shah
    • 1
  • P.R. Vyas
    • 1
  • H.S. Chhatpar
    • 1
  1. 1.Department of Microbiology and Biotechnology Centre, Faculty of ScienceMS University of Baroda, Baroda –GujaratIndia