Mycopathologia

, Volume 152, Issue 2, pp 103–108

The occurrence of culmorin and hydroxy-culmorins in cereals

  • Mebrat Ghebremeskel
  • Wenche Langseth
Article

DOI: 10.1023/A:1012479823193

Cite this article as:
Ghebremeskel, M. & Langseth, W. Mycopathologia (2001) 152: 103. doi:10.1023/A:1012479823193

Abstract

Forty-five samples from 1988–1995 of naturally contaminated grain, barley, wheat and oats, three samples of mixed feed, and 16 samples of grain artificially inoculated with Fusarium culmorum during the flowering stage were analysed for deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-acetyl-DON), culmorin and hydroxy-culmorins. These compounds are secondary metabolites produced by the fungal species F. culmorum and F. graminearum. Acetonitrile-water extract of the samples was purified on a MycosepTM#225 column, derivetized using pentafluoropropionic anhydride (PFPA) and analysed by gas chromatography-mass spectrometry (GC-MS). The amount of each of culmorin, 5-, 12-, 14 and 15-hydroxy-culmorin and one unknown hydroxy-culmorin were determined relative to the amount of DON plus 3-acetyl DON for each sample. The ratio between the total amount of culmorin compounds and the DON compounds ranged from 0.14 to 1.07 in the samples. This study shows that there is a strong correlation between the amount of DON present in the grain and the amount of culmorin and hydroxy-culmorins present. The ratio of each of the culmorin compounds relative to the amount of DON compounds were in the same range in the grain artificially inoculated by F. culmorum as found in an earlier study for F. culmorum strains cultivated on rice, while the hydroxy-culmorin profile in the naturally contaminated grain was more similar to what was found for the F. graminearum cultures in the same study [1]. These results indicate that F. graminearum may be a relatively important source for DON in grain also in relatively cold areas.

deoxynivalenol Fusarium culmorumgas chromatographygraingraminearummass spectrometry

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Mebrat Ghebremeskel
    • 1
  • Wenche Langseth
    • 1
  1. 1.Department of ChemisatryNational Veterinary InstituteOsloNorway