Chromosome Research

, Volume 9, Issue 7, pp 569–584

Arrangements of macro- and microchromosomes in chicken cells

Authors

  • Felix A. Habermann
    • Institute of Anthropology and Human GeneticsUniversity of Munich (LMU)
  • Marion Cremer
    • Institute of Anthropology and Human GeneticsUniversity of Munich (LMU)
  • Joachim Walter
    • Institute of Anthropology and Human GeneticsUniversity of Munich (LMU)
  • Gregor Kreth
    • Kirchhoff-Institute of PhysicsUniversity of Heidelberg
  • Johann von Hase
    • Kirchhoff-Institute of PhysicsUniversity of Heidelberg
  • Karin Bauer
    • Institute of Anthropology and Human GeneticsUniversity of Munich (LMU)
  • Johannes Wienberg
    • Institute of Anthropology and Human GeneticsUniversity of Munich (LMU)
  • Christoph Cremer
    • Kirchhoff-Institute of PhysicsUniversity of Heidelberg
  • Thomas Cremer
    • Institute of Anthropology and Human GeneticsUniversity of Munich (LMU)
  • Irina Solovei
    • Institute of Anthropology and Human GeneticsUniversity of Munich (LMU)
Article

DOI: 10.1023/A:1012447318535

Cite this article as:
Habermann, F.A., Cremer, M., Walter, J. et al. Chromosome Res (2001) 9: 569. doi:10.1023/A:1012447318535

Abstract

Arrangements of chromosome territories in nuclei of chicken fibroblasts and neurons were analysed employing multicolour chromosome painting, laser confocal scanning microscopy and three-dimensional (3D) reconstruction. The chicken karyotype consists of 9 pairs of macrochromosomes and 30 pairs of microchromosomes. Although the latter represent only 23% of the chicken genome they contain almost 50% of its genes. We show that territories of microchromosomes in fibroblasts and neurons were clustered within the centre of the nucleus, while territories of the macrochromosomes were preferentially located towards the nuclear periphery. In contrast to these highly consistent radial arrangements, the relative arrangements of macrochromosome territories with respect to each other (side-by-side arrangements) were variable. A stringent radial arrangement of macro- and microchromosomes was found in mitotic cells. Replication labelling studies revealed a pattern of DNA replication similar to mammalian cell nuclei: gene dense, early replicating chromatin mostly represented by microchromosomes, was located within the nuclear interior, surrounded by a rim of late replicating chromatin. These results support the evolutionary conservation of several features of higher-order chromatin organization between mammals and birds despite the differences in their karyotypes.

chicken interphase nucleichromosome arrangement3D M-FISHnuclear architecture
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Copyright information

© Kluwer Academic Publishers 2001