Journal of Neuro-Oncology

, Volume 53, Issue 2, pp 87–98

Dynamics of C6 Astrocytoma Invasion into Three-dimensional Collagen Gels

  • Rolando Del Maestro
  • Richard Shivers
  • Warren McDonald
  • Adrian Del Maestro
Article

DOI: 10.1023/A:1012236830230

Cite this article as:
Del Maestro, R., Shivers, R., McDonald, W. et al. J Neurooncol (2001) 53: 87. doi:10.1023/A:1012236830230

Abstract

Objective: The dynamic mechanisms underlying the three-dimensional invasive paradigm of C6 astrocytoma cells has been assessed.

Methods: Spheroids of C6 astrocytoma cells were implanted into three-dimensional collagen type I gels (vitrogen 100) and individual C6 astrocytoma cell invasion monitored. Time-lapsed videomicroscopy was used to assess the dynamic components of cell invasion in three dimensions while scanning and transmission electron microscopy were used to assess matrix architecture and the static aspects of cell invasion.

Results: Videomicroscopy outlined an invasion paradigm continuum with repeating phases. A cell surface ruffling phase was followed by invadopodia extension and pull up phases. For some cells the collagen type I matrix extracellular matrix appeared to modify the ability of C6 cells to carry out their invasion paradigm.

Conclusions: C6 astrocytoma cells invading a three-dimensional collagen type I matrix utilize a invasion paradigm made up of a number of phases. A better understanding of the dynamic invasion paradigms of malignant glial cells may be useful in the development of effective treatment strategies to prevent or modify malignant glioma invasion.

C6 astrocytoma spheroids collagen type I gel glioma three-dimensional invasion time-lapsed videomicroscopy 

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Rolando Del Maestro
    • 1
  • Richard Shivers
    • 2
  • Warren McDonald
    • 3
  • Adrian Del Maestro
    • 3
  1. 1.Brain Tumour Research CentreMontreal Neurological Institute and HospitalMontrealCanada
  2. 2.Department of ZoologyUniversity of Western OntarioCanada
  3. 3.Brain Tumour Research Laboratories, Division of NeurosurgeryUniversity of Western OntarioCanada