Pharmaceutical Research

, Volume 14, Issue 5, pp 578–590

Thermophysical Properties of Trehalose and Its Concentrated Aqueous Solutions

  • Danforth P. Miller
  • Juan J. de Pablo
  • Horacio Corti

DOI: 10.1023/A:1012192725996

Cite this article as:
Miller, D.P., de Pablo, J.J. & Corti, H. Pharm Res (1997) 14: 578. doi:10.1023/A:1012192725996


Purpose. To address the lack of fundamental thermophysical data for trehalose and its aqueous systems by measuring equilibrium and non-equilibrium properties of such systems.

Methods/Results. Differential scanning calorimetry (DSC) and dynamic mechanical analysis were used to measure glass transition temperatures of trehalose and its solutions. X-ray diffractometry was used to verify the structure of amorphous trehalose. Controlled-stress rheometry was used to measure viscosity of several aqueous trehalose systems at ambient and sub-ambient temperatures. Over this temperature range, the density of these solutions was also measured with a vibrating tube densimeter. The equilibrium phase diagram of aqueous trehalose was determined by measuring the solubility and freezing point depression.

Conclusions. Our solubility measurements, which have allowed long times for attainment of chemical equilibrium, are substantially different from those reported earlier that used different techniques. Our measurements of the glass transition temperature of trehalose are higher than reported values. A simple model for the glass transition is presented to describe our experimental observations.

trehalose phase diagram glass transition viscosity molar volume cryoprotection 

Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • Danforth P. Miller
    • 1
  • Juan J. de Pablo
    • 1
  • Horacio Corti
    • 2
  1. 1.Department of Chemical EngineeringUniversity of Wisconsin-MadisonMadison
  2. 2.Departamento de Química, C.N.E.A. and Institute de Química de MaterialesMedio Ambiente y EnergíaBuenos AiresArgentina

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