Barley somaclones associated with high yield or resistance to powdery mildew
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
A study was conducted to evaluate the usefulness of somaclonal variation as a means to obtain powdery mildew resistance in the background of an agronomically elite, high-yielding barley (Hordeum vulgare L.) cultivar. A total of 170 Ro-derived lines were regenerated from embryo-induced callus of the barley cultivar Léger. Forty-five lines were selected and evaluated in replicated field plots at two locations in Eastern Canada. In comparison with Léger, one of the 45 lines was higher yielding, one produced a greater test weight, two had a greater seed weight, and one was shorter in plant height. Three lines were found to segregate for resistance to powdery mildew (Erysiphe graminis DC ex Merat f. sp. hordei EM). Many of the single-plant selections from the three resistant lines showed resistance to powdery mildew under field conditions for two years. Three lines were eventually promoted to the official registration tests in Ontario. One of the three lines was subsequently registered as a new cultivar (AC Malone) in Canada. To our knowledge, AC Malone is the world's first barley cultivar selected from somaclonal variation. The results of this study demonstrate that it is possible to obtain high- yielding or disease-resistant lines from callus culture in barley.
- Ahloowalia, B.S., 1987. Plant regeneration from embryo-callus culture in barley. Euphytica36: 659–665. CrossRef
- Baillie, A.M.R, B.G. Rossnagel & K.K. Kartha, 1992. Field evaluation of barley (Hordeumvulgare L.) genotypes derived from tissue culture. Can J Plant Sci 72: 725–733.
- Brar, D.S. & S.M. Jain, 1998.Somaclonal variation: mechanism and applications in crop improvement. In: S.M. Jain, D.S. Brar & B.S. Ahloowalia (Eds.), Somaclonal Variation and Induced Mutations in Crop Improvement, pp. 15–37. Kluwer Academic Publishers, Dordrecht.
- Bregitzer, P. & M. Poulson, 1995. Agronomic performance of barley lines derived fromtissue culture. Crop Sci 35: 1144–1148. CrossRef
- Bregitzer, P., M. Poulson & B.L. Jones, 1995. Malting qualityof barley lines derived from tissue culture. Cereal Chem 72: 433–435.
- Breiman, A. & D. Rotem-Abarbanell,1990. Somaclonal variation in barley (Hordeum vulgare L.). In: Y.P.S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry, Vol. II. Somaclonal Variation in Crop Improvement I, pp. 352–375. Springer-Verlag, Berlin.
- Choo, T.M., J.C. Li, R.A. Martin & K.M. Ho, 2000. AC Malone barley. Can J Plant Sci 80: 597–598.
- Dunwell, J.M., M. Cornish, W. Powell & E.M. Borrino, 1986. An evaluation of the field performance of the progeny of plants regenerated from embryos of Hordeum vulgare cv. Golden Promise. J Agric Sci, Camb 107: 561–564. CrossRef
- Falk, D.E., L.A. Hunt, G. Meatherall & Z. Szlavnics, 1996. Ontario Recommendation Trials: 1995 ProgressReport on Oats, Barley, and Wheat. Department of Crop Science, University of Guelph, Guelph, Ontario.
- Fejer, S.O.,G. Fedak & K.M. Ho, 1984. Léger barley. Can J Plant Sci 64: 195–196. CrossRef
- Gaponenko, A.K.,T.F. Petrova, A.R. Iskakov & A.A. Sozinov, 1988. Cytogenetics of in vitro cultured somatic cells and regenerated plants of barley (Hordeum vulgare L.). Theor Appl Genet 75: 905–911.
- Gozukirmizi, N., S. Ari, G. Oraler, Y. Okatan & N. Unsal, 1990. Callus induction, plant regeneration and chromosomal variations in barley. Acta Bot Neerl 39: 379–387.
- Hanzel, J.J., J.P. Miller, M.A. Brinkman & E. Fendos, 1985. Genotype and mediaeffects on callus formation and regeneration in barley. Crop Sci 25: 27–31. CrossRef
- Kaeppler, S.M. & R.L. Phillips,1993. Tissue culture-induced DNA methylation variation in maize. Proc Natl Acad Sci 90: 8773–8776. CrossRef
- Karp, A., 1995. Somaclonal variation as a tool for crop improvement. Euphytica 85: 295–302. CrossRef
- Karp, A.,S.H. Steele, S. Parmar, M.G.K. Jones, P.R. Shewry & A. Breiman, 1987. Relative stability among barley plants regenerated from cultured immature embryos. Genome 29: 405–412.
- Luckett, D.J., D. Rose & E. Knights,1989. Paucity of somaclonal variation from immature embryo culture of barley. Aust J Agric Res 40: 1155–1159. CrossRef
- Lupotto, E., 1984. Callus induction and plant regeneration from mature embryos. Ann Bot 54: 523–529.
- Mohanty, B.D. & P.D. Ghosh, 1988. Somatic embryogenesis and plant regeneration from leaf callus ofHordeum vulgare. AnnBot 61: 551–555.
- Pickering, R.A., 1989. Plant regeneration and variants from calliderived from immature embryos of diploid barley (Hordeum vulgare L.) and H. vulgare ×H. bulbosum L. crosses. Theor Appl Genet 78: 105–122. CrossRef
- Steel, R.G.D. & J.H. Torrie, 1980. Principles and Procedures of Statistics.2nd edition. McGraw-Hill Book Company, New York, NY.
- Todorovska, E., A. Trifonova, M. Petrova, Z. Vitanova, E. Marinova, M. Gramatikova, D. Valcheva, S. Zaprianov, N. Mersinkov & A. Atanassov, 1997. Agronomic performance and molecular assessment of tissue culture-derived barley lines. Plant Breeding 116: 511–517. CrossRef
- Ullrich, S.E., J.M. Edmiston, A. Kleinhofs, D.A. Kudrna & M.E.H. Maatougui, 1991. Evaluation ofsomaclonal variation in barley. Cereal Res Comm 19: 245–260.
- Veilleux, R.E. & A.A.T. Johnson, 1998.Somaclonal variation: molecular analysis, transformation interaction, and utilization. Plant Breed Reviews 16: 229–268.
- Wenzel, G. & B. Foroughi-Wehr, 1990. Progeny tests of barley, wheat, and potato regenerated from cellcultures after in vitro selection for disease resistance. Theor Appl Genet 80: 359–365. CrossRef
- Yuan, M., Y. Zhang, M. Zhu, A. Xu & H. Pei, 1991. Cytogenetic studies on barley plants regenerated from immature embryo culture and their progenies. Acta Agron Sinica 17: 171–177.
- Barley somaclones associated with high yield or resistance to powdery mildew
Volume 121, Issue 3 , pp 349-356
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- high yield
- Hordeum vulgare
- powdery mildew
- somaclonal variation
- Industry Sectors
- Author Affiliations
- 1. Biology Institute of Shanxi, Taiyuan, Shanxi Province, China, 030006
- 2. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada, K1A 0C6
- 3. Department of Crop Science, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
- 4. Atlantic Food and Horticulture Research Centre, Agriculture and Agri-Food Canada, Kentville, Nova Scotia, Canada, B4N 5E3