Article

Digestive Diseases and Sciences

, Volume 46, Issue 10, pp 2179-2186

First online:

Differential Expression of Cytoplasmic Carbonic Anhydrases, CA I and II, and Membrane-Associated Isozymes, CA IX and XII, in Normal Mucosa of Large Intestine and in Colorectal Tumors

  • Antti J. KivelãAffiliated withDepartment of Anatomy and Cell Biology, University of Oulu
  • , Juha SaarnioAffiliated withDepartment of Surgery, University of Oulu
  • , Tuomo J. KarttunenAffiliated withDepartment of Surgery, University of Oulu
  • , Jyrki KivelãAffiliated withDepartment of Anatomy and Cell Biology, University of OuluParolannummi Garrison Hospital
  • , Anna-Kaisa ParkkilaAffiliated withDepartment of Anatomy and Cell Biology, University of OuluDepartment of Surgery, University of Oulu
  • , Silvia PastorekovaAffiliated withDepartment of Molecular Biology, Institute of Virology, Slovak Academy of Sciences
  • , Jaromir PastorekAffiliated withDepartment of Molecular Biology, Institute of Virology, Slovak Academy of Sciences
  • , Abdul WaheedAffiliated withDepartment of Biochemistry and Molecular Biology, Saint Louis University School of Medicine
  • , William S. SlyAffiliated withDepartment of Biochemistry and Molecular Biology, Saint Louis University School of Medicine
    • , Seppo ParkkilaAffiliated withDepartment of Anatomy and Cell Biology, University of Oulu
    • , Hannu RajaniemiAffiliated withDepartment of Anatomy and Cell Biology, University of Oulu

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Abstract

This study compares the localization of carbonic anhydrase isozymes (CA) I and II and that of IX and XII in normal large intestine and in colorectal tumors. Immunohistochemical studies were performed on 69 colorectal lesions. While the normal mucosa of the large intestine showed high expression for CA I and II, the intensity of the immunostaining for both isozymes decreased in benign lesions and was very weak in malignant tumors. The reciprocal pattern of expression observed for these cytoplasmic isozymes and transmembrane CA IX and XII in intestinal tissue specimens supports the suggestion that CA IX and XII may be functionally involved in tumor progression to malignancy and/or in invasion. By contrast, while CA I and II are prominent in normal colorectal mucosa, where they play a role in regulation of pH homeostasis and water and ion transport, loss of expression of these cytoplasmic isozymes consistently accompanies progression to malignant transformation.

adenoma cancer carbonic anhydrase gastrointestinal gut human