Digestive Diseases and Sciences

, Volume 46, Issue 10, pp 2179–2186

Differential Expression of Cytoplasmic Carbonic Anhydrases, CA I and II, and Membrane-Associated Isozymes, CA IX and XII, in Normal Mucosa of Large Intestine and in Colorectal Tumors

  • Antti J. Kivelã
  • Juha Saarnio
  • Tuomo J. Karttunen
  • Jyrki Kivelã
  • Anna-Kaisa Parkkila
  • Silvia Pastorekova
  • Jaromir Pastorek
  • Abdul Waheed
  • William S. Sly
  • Seppo Parkkila
  • Hannu Rajaniemi
Article

DOI: 10.1023/A:1011910931210

Cite this article as:
Kivelã, A.J., Saarnio, J., Karttunen, T.J. et al. Dig Dis Sci (2001) 46: 2179. doi:10.1023/A:1011910931210

Abstract

This study compares the localization of carbonic anhydrase isozymes (CA) I and II and that of IX and XII in normal large intestine and in colorectal tumors. Immunohistochemical studies were performed on 69 colorectal lesions. While the normal mucosa of the large intestine showed high expression for CA I and II, the intensity of the immunostaining for both isozymes decreased in benign lesions and was very weak in malignant tumors. The reciprocal pattern of expression observed for these cytoplasmic isozymes and transmembrane CA IX and XII in intestinal tissue specimens supports the suggestion that CA IX and XII may be functionally involved in tumor progression to malignancy and/or in invasion. By contrast, while CA I and II are prominent in normal colorectal mucosa, where they play a role in regulation of pH homeostasis and water and ion transport, loss of expression of these cytoplasmic isozymes consistently accompanies progression to malignant transformation.

adenomacancercarbonic anhydrasegastrointestinalguthuman

Copyright information

© Plenum Publishing Corporation 2001

Authors and Affiliations

  • Antti J. Kivelã
    • 1
  • Juha Saarnio
    • 2
  • Tuomo J. Karttunen
    • 2
  • Jyrki Kivelã
    • 1
    • 3
  • Anna-Kaisa Parkkila
    • 1
    • 2
  • Silvia Pastorekova
    • 4
  • Jaromir Pastorek
    • 4
  • Abdul Waheed
    • 5
  • William S. Sly
    • 5
  • Seppo Parkkila
    • 1
  • Hannu Rajaniemi
    • 6
  1. 1.Department of Anatomy and Cell BiologyUniversity of OuluOuluFinland
  2. 2.Department of SurgeryUniversity of OuluOuluFinland
  3. 3.Parolannummi Garrison HospitalHattulaFinland
  4. 4.Department of Molecular Biology, Institute of VirologySlovak Academy of SciencesBratislavaSlovak Republic
  5. 5.Department of Biochemistry and Molecular BiologySaint Louis University School of MedicineSt. LouisUSA
  6. 6.Department of Anatomy and Cell BiologyUniversity of OuluOuluFinland