Photosynthesis Research

, Volume 68, Issue 2, pp 175–180

Preparation and functional characterization of thylakoids from Arabidopsis thaliana

Authors

  • Anna Paola Casazza
    • Dept of BiologyUniversity of Milano
  • Delia Tarantino
    • Dept of BiologyUniversity of Milano
    • Dept of BiologyUniversity of Milano
Article

DOI: 10.1023/A:1011818021875

Cite this article as:
Casazza, A.P., Tarantino, D. & Soave, C. Photosynthesis Research (2001) 68: 175. doi:10.1023/A:1011818021875

Abstract

A protocol for the isolation of functional thylakoids from Arabidopsis thaliana leaves was developed. The critical factor in obtaining active, coupled and stable preparation is the inclusion of EDTA and EGTA in the grinding buffer. Preparations were characterized with respect to the whole or partial electron transport chain, ATP/NADPH, ATP/O2 and PS II/chlorophyll ratios. Sensitivity to a light-chill photoinhibitory treatment was also determined by evaluating the decrease in both maximal photochemical efficiency (Fv/Fm) and in electron transport rate.

Copyright information

© Kluwer Academic Publishers 2001