Chromosome Research

, Volume 9, Issue 6, pp 469–473

Additional locus of rDNA sequence specific to the X chromosome of the liverwort, Marchantia polymorpha

  • Shigeki Nakayama
  • Mariko Fujishita
  • Takefumi Sone
  • Kanji Ohyama
Article

DOI: 10.1023/A:1011676328165

Cite this article as:
Nakayama, S., Fujishita, M., Sone, T. et al. Chromosome Res (2001) 9: 469. doi:10.1023/A:1011676328165

Abstract

The molecular cytogenetic organization of 17S ribosomal RNA genes (17S rDNA), a part of the 45S rDNA repeat, was investigated on the chromosomes of the liverwort Marchantia polymorpha using fluorescence in-situ hybridization (FISH). The numbers of 17S rDNA loci visualized in female and male chromosomes were ten and nine, respectively. This heterogeneous localization was due to the presence of an additional 17S rDNA locus on the X chromosome and its absence on the Y chromosome. The signal on the X chromosome covered almost the entire region of its long arm. The other nine signals were observed on the same loci of respective autosomes in both sexes. Southern hybridization analysis revealed an additional band including 17S rDNA exclusively on EcoRI digested female genomic DNA supporting the existence of an additional 17S rDNA locus on the X chromosome.

fluorescence in-situ hybridization (FISH) Marchantia polymorpha ribosomal RNA genes (rDNA) sex chromosomes 

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Shigeki Nakayama
    • 1
  • Mariko Fujishita
    • 1
  • Takefumi Sone
    • 3
  • Kanji Ohyama
    • 3
  1. 1.National Institute of Agrobiological ResourcesTsukubaJapan
  2. 2.Bio-oriented Technology Research Advancement InstitutionTokyoJapan
  3. 3.Graduate School of BiostudiesKyoto UniversityKyotoJapan