Biotechnology Letters

, Volume 23, Issue 17, pp 1385–1389

Hepatocyte-specific porous polymer-scaffolds of alginate/galactosylated chitosan sponge for liver-tissue engineering

  • Jun Yang
  • Teak Woong Chung
  • Masato Nagaoka
  • Mitsuaki Goto
  • Chong-Su Cho
  • Toshihiro Akaike
Article

DOI: 10.1023/A:1011600414225

Cite this article as:
Yang, J., Woong Chung, T., Nagaoka, M. et al. Biotechnology Letters (2001) 23: 1385. doi:10.1023/A:1011600414225

Abstract

Porous scaffolds of alginate/galactosylated chitosan (ALG/GC) sponges were prepared by lyophilization for liver-tissue engineering. Primary hepatocytes in ALG/GC sponges showed higher cell attachment and viability than in alginate alone owing to the specific interaction of the asialoglycoprotein receptors on hepatocyte with the galactose residues on ALG/GC sponges. Improvements in spheroid formation and long-term liver-specific functions of the immobilized hepatocyte were also observed in ALG/GC sponge.

alginategalactosylated chitosanhepatocyteliver-tissue engineeringsponge

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Jun Yang
    • 1
  • Teak Woong Chung
    • 2
  • Masato Nagaoka
    • 1
  • Mitsuaki Goto
    • 2
  • Chong-Su Cho
    • 3
  • Toshihiro Akaike
    • 1
  1. 1.Department of Biomolecular EngineeringTokyo Institute of TechnologyYokohamaJapan
  2. 2.Bio Quest Research Co. Ltd.TokyoJapan
  3. 3.School of Agricultural BiotechnologySeoul National UniversitySuwonKorea