Hepatocyte-specific porous polymer-scaffolds of alginate/galactosylated chitosan sponge for liver-tissue engineering
10.1023/A:1011600414225 Cite this article as: Yang, J., Woong Chung, T., Nagaoka, M. et al. Biotechnology Letters (2001) 23: 1385. doi:10.1023/A:1011600414225 Abstract
Porous scaffolds of alginate/galactosylated chitosan (ALG/GC) sponges were prepared by lyophilization for liver-tissue engineering. Primary hepatocytes in ALG/GC sponges showed higher cell attachment and viability than in alginate alone owing to the specific interaction of the asialoglycoprotein receptors on hepatocyte with the galactose residues on ALG/GC sponges. Improvements in spheroid formation and long-term liver-specific functions of the immobilized hepatocyte were also observed in ALG/GC sponge.
alginate galactosylated chitosan hepatocyte liver-tissue engineering sponge References
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