Adsorption of vascular endothelial growth factor to two different apatitic materials and its release

  • V. Midy
  • E. Hollande
  • C. Rey
  • M. Dard
  • J. Ploue¨t
Article

DOI: 10.1023/A:1011286818733

Cite this article as:
Midy, V., Hollande, E., Rey, C. et al. Journal of Materials Science: Materials in Medicine (2001) 12: 293. doi:10.1023/A:1011286818733

Abstract

The aim of our study was to assess the ability of calcium phosphate powders to serve as growth factor carriers. Vascular endothelial growth factor (VEGF), in particular, is locally involved in the bone formation process throughout osteoblast differentiation. Two different apatitic substrates were tested: hydroxyapatite (HA), widely used as biomaterial, and nanocrystalline carbonated apatite (CA), which has a composition similar to bone mineral crystals. These materials have been compared for their VEGF adsorption and release properties. The adsorption of the growth factor was higher on CA than on HA probably due to differences of both the proteins and the powders involved. The specific activity of the VEGF released was also tested to determine the available activity for cells in contact with these materials. Interestingly, the bioactivity of the VEGF released from CA quantified on fetal bovine aortic endothelial cells (FBAE) by evaluating the proliferation activity, exhibited no marked difference compared to native VEGF. Qualitatively, VEGF adsorbed on CA material induced well-defined collagen type I immunostaining on osteoblast cells compared to the staining obtained after VEGF adsorption on HA. © 2001 Kluwer Academic Publishers

Download to read the full article text

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • V. Midy
    • 1
    • 2
  • E. Hollande
    • 1
  • C. Rey
    • 2
  • M. Dard
    • 3
  • J. Ploue¨t
    • 4
  1. 1.Laboratoire de Cytophysiologie des cellules eucaryotesToulouseFrance
  2. 2.Laboratoire des Mate´riaux et Interfaces, Phosphates et Biomate´riaux, UPRESA CNRS-5071ToulouseFrance
  3. 3.Merck Biomaterial ResearchDarmstadtGermany
  4. 4.Laboratoire de Biologie Mole´culaire des Eucaryotes, UPR CNRS 9006ToulouseFrance

Personalised recommendations