Cross-linking by 1-ethyl-3- (3-dimethylaminopropyl)-carbodiimide (EDC) of a collagen/elastin membrane meant to be used as a dermal substitute: effects on physical, biochemical and biological features in vitro

  • B. Hafemann
  • K. Ghofrani
  • H.-G. Gattner
  • H. Stieve
  • N. Pallua
Article

DOI: 10.1023/A:1011205221972

Cite this article as:
Hafemann, B., Ghofrani, K., Gattner, HG. et al. Journal of Materials Science: Materials in Medicine (2001) 12: 437. doi:10.1023/A:1011205221972

Abstract

Next to in vitro-cultured autogeneic keratinocytes for the restoration of epidermis, a suitable dermal matrix is a mandatory component of an artificial skin substitute for the permanent covering of full thickness skin defects. In our model a xenogeneic membrane, consisting of processed native collagen and elastin of porcine origin is meant to serve as a template for the formation of a neo-dermis. In order to improve the resistance of this matrix against enzymatical degradation, we cross-linked it by using the carbodiimide 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) together with N-hydroxysuccinimide. Chemical cross-linking by these agents at two different degrees (shrinkage temperatures 63 °C and 81 °C) had no relevant effect on mechanical features or water-uptake capacity. The time needed for enzymatic digestion was increased by cross-linking. Concerning growth and spreading of fibroblasts and keratinocytes on and within the structure of this membrane, we did not observe a difference between cross-linked and non-cross-linked material (shrinkage temperature 48 °C). We therefore expect that cross-linking by EDC is an effective means to control the degradation of the collagen/elastin membranes in vivo without a significant influence on their biocompatibility. © 2001 Kluwer Academic Publishers

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • B. Hafemann
    • 1
  • K. Ghofrani
    • 2
  • H.-G. Gattner
    • 3
  • H. Stieve
    • 4
  • N. Pallua
    • 1
  1. 1.Clinic of Plastic Surgery, Hand and Burn SurgeryUniversity of TechnologyAachenGermany
  2. 2.Interdisciplinary Center for Clinical Research BiomatUniversity of TechnologyAachenGermany
  3. 3.Institute of Textile and Macromolecular ChemistryUniversity of TechnologyAachenGermany
  4. 4.Institute of Biology IIUniversity of TechnologyAachenGermany

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