Journal of Applied Phycology

, Volume 13, Issue 2, pp 135–142

An axenic cyclostat of Prochlorococcus PCC 9511 with a simulator of natural light regimes

Authors

  • F. Bruyant
    • Laboratoire de Physique et Chimie MarinesUniversité Pierre et Marie Curie and CNRS
  • M. Babin
    • Laboratoire de Physique et Chimie MarinesUniversité Pierre et Marie Curie and CNRS
  • A. Sciandra
    • Laboratoire d'Océanographie Biologique et Ecologie du Plancton MarinUniversité Pierre et Marie Curie and CNRS
  • D. Marie
    • Station BiologiqueUniversité Pierre et Marie Curie and CNRS
  • B. Genty
    • Laboratoire d'Ecophysiologie VégétaleUniversité Paris Sud and CNRS
  • H. Claustre
    • Laboratoire de Physique et Chimie MarinesUniversité Pierre et Marie Curie and CNRS
  • J. Blanchot
    • Station BiologiqueUniversité Pierre et Marie Curie and CNRS
  • A. Bricaud
    • Laboratoire de Physique et Chimie MarinesUniversité Pierre et Marie Curie and CNRS
  • R. Rippka
    • Département de Biochimie et Génétique Moléculaire, Institut PasteurUnité de Physiol Microbienne (CNRS URA 1129)
  • S. Boulben
    • Station BiologiqueUniversité Pierre et Marie Curie and CNRS
  • F. Louis
    • Laboratoire de Physique et Chimie MarinesUniversité Pierre et Marie Curie and CNRS
  • F. Partensky
    • Station BiologiqueUniversité Pierre et Marie Curie and CNRS
Article

DOI: 10.1023/A:1011144310988

Cite this article as:
Bruyant, F., Babin, M., Sciandra, A. et al. Journal of Applied Phycology (2001) 13: 135. doi:10.1023/A:1011144310988

Abstract

A cyclostat was designed for growing the oceanic oxyphotobacterium Prochlorococcus PCC 9511. Culture of this organism, known to bedifficult to grow, was mastered for a large volume. Prochlorococcusgrew well and axenic conditions were maintained for up to 15 days. Wedesigned an illumination system allowing a smooth bell-shaped irradiancecurve reaching almost 1000 μmol quanta m-2 s-1 tobe obtained. Cell division was strongly synchronised under theseillumination conditions, which were close to those found at low latitude inthe upper layer of ocean. The described device is particularly well suited tomake experiments requiring up to 6 L per day of well synchronised,exponentially-growing Prochlorococcus culture.

cyclostatProchlorococcuscultureaxeniccyclephytoplankton

Copyright information

© Kluwer Academic Publishers 2001