Article

Journal of Applied Phycology

, Volume 13, Issue 2, pp 135-142

First online:

An axenic cyclostat of Prochlorococcus PCC 9511 with a simulator of natural light regimes

  • F. BruyantAffiliated withLaboratoire de Physique et Chimie Marines, Université Pierre et Marie Curie and CNRS
  • , M. BabinAffiliated withLaboratoire de Physique et Chimie Marines, Université Pierre et Marie Curie and CNRS
  • , A. SciandraAffiliated withLaboratoire d'Océanographie Biologique et Ecologie du Plancton Marin, Université Pierre et Marie Curie and CNRS
  • , D. MarieAffiliated withStation Biologique, Université Pierre et Marie Curie and CNRS
  • , B. GentyAffiliated withLaboratoire d'Ecophysiologie Végétale, Université Paris Sud and CNRS
  • , H. ClaustreAffiliated withLaboratoire de Physique et Chimie Marines, Université Pierre et Marie Curie and CNRS
  • , J. BlanchotAffiliated withStation Biologique, Université Pierre et Marie Curie and CNRS
  • , A. BricaudAffiliated withLaboratoire de Physique et Chimie Marines, Université Pierre et Marie Curie and CNRS
  • , R. RippkaAffiliated withDépartement de Biochimie et Génétique Moléculaire, Institut Pasteur, Unité de Physiol Microbienne (CNRS URA 1129)
    • , S. BoulbenAffiliated withStation Biologique, Université Pierre et Marie Curie and CNRS
    • , F. LouisAffiliated withLaboratoire de Physique et Chimie Marines, Université Pierre et Marie Curie and CNRS
    • , F. PartenskyAffiliated withStation Biologique, Université Pierre et Marie Curie and CNRS

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Abstract

A cyclostat was designed for growing the oceanic oxyphotobacterium Prochlorococcus PCC 9511. Culture of this organism, known to bedifficult to grow, was mastered for a large volume. Prochlorococcusgrew well and axenic conditions were maintained for up to 15 days. Wedesigned an illumination system allowing a smooth bell-shaped irradiancecurve reaching almost 1000 μmol quanta m-2 s-1 tobe obtained. Cell division was strongly synchronised under theseillumination conditions, which were close to those found at low latitude inthe upper layer of ocean. The described device is particularly well suited tomake experiments requiring up to 6 L per day of well synchronised,exponentially-growing Prochlorococcus culture.

cyclostat Prochlorococcus culture axenic cycle phytoplankton