Pharmaceutical Research

, Volume 18, Issue 6, pp 859–866

Ritonavir: An Extraordinary Example of Conformational Polymorphism

  • John Bauer
  • Stephen Spanton
  • Rodger Henry
  • John Quick
  • Walter Dziki
  • William Porter
  • John Morris
Article

DOI: 10.1023/A:1011052932607

Cite this article as:
Bauer, J., Spanton, S., Henry, R. et al. Pharm Res (2001) 18: 859. doi:10.1023/A:1011052932607

Abstract

Purpose. In the summer of 1998, Norvir semi-solid capsules supplies were threatened as a result of a new much less soluble crystal form of ritonavir. This report provides characterization of the two polymorphs and the structures and hydrogen bonding network for each form.

Methods. Ritonavir polymorphism was investigated using solid state spectroscopy and microscopy techniques including solid state NMR, Near Infrared Spectroscopy, powder X-ray Diffraction and Single crystal X-ray. A sensitive seed detection test was developed.

Results. Ritonavir polymorphs were thoroughly characterized and the structures determined. An unusual conformation was found for form II that results in a strong hydrogen bonding network A possible mechanism for heterogeneous nucleation of form II was investigated.

Conclusions. Ritonavir was found to exhibit conformational polymorphism with two unique crystal lattices having significantly different solubility properties. Although the polymorph (form II) corresponding to the “cis” conformation is a more stable packing arrangement, nucleation, even in the presence of form II seeds, is energetically unfavored except in highly supersaturated solutions. The coincidence of a highly supersaturated solution and a probable heterogeneous nucleation by a degradation product resulted in the sudden appearance of the more stable form II polymorph.

polymorphismcrystal formsritonavirNorvircarbamateAIDS drug

Copyright information

© Plenum Publishing Corporation 2001

Authors and Affiliations

  • John Bauer
    • 1
  • Stephen Spanton
    • 1
  • Rodger Henry
    • 1
  • John Quick
    • 1
  • Walter Dziki
    • 1
  • William Porter
    • 1
  • John Morris
    • 1
  1. 1.Pharmaceutical Products DivisionAbbott LaboratoriesNorth Chicago