Neurochemical Research

, Volume 26, Issue 3, pp 187–190

Distribution of [3H]GR65630 Binding in Human Brain Postmortem

Authors

  • Donatella Marazziti
    • Dipartimento di Psichiatria, Neurobiologia, Farmacologia e Biotecnologie, Clinica PsichiatricaUniversity of Pisa
  • Laura Betti
    • Dipartimento di Psichiatria, Neurobiologia, Farmacologia e Biotecnologie, Clinica PsichiatricaUniversity of Pisa
  • Gino Giannaccini
    • Dipartimento di Psichiatria, Neurobiologia, Farmacologia e Biotecnologie, Clinica PsichiatricaUniversity of Pisa
  • Alessandra Rossi
    • Dipartimento di Psichiatria, Neurobiologia, Farmacologia e Biotecnologie, Clinica PsichiatricaUniversity of Pisa
  • Irene Masala
    • Dipartimento di Psichiatria, Neurobiologia, Farmacologia e Biotecnologie, Clinica PsichiatricaUniversity of Pisa
  • Stefano Baroni
    • Dipartimento di Psichiatria, Neurobiologia, Farmacologia e Biotecnologie, Clinica PsichiatricaUniversity of Pisa
  • Giovanni B. Cassano
    • Dipartimento di Psichiatria, Neurobiologia, Farmacologia e Biotecnologie, Clinica PsichiatricaUniversity of Pisa
  • Antonio Lucacchini
    • Dipartimento di Psichiatria, Neurobiologia, Farmacologia e Biotecnologie, Clinica PsichiatricaUniversity of Pisa
Article

DOI: 10.1023/A:1010939530412

Cite this article as:
Marazziti, D., Betti, L., Giannaccini, G. et al. Neurochem Res (2001) 26: 187. doi:10.1023/A:1010939530412

Abstract

We investigated the distribution of serotonin (5-HT) receptors of type 3 (5-HT3) in human brain areas, by means of the the specific binding of [3H]GR65630. The brains were obtained during autoptic sessions from 6 subjects. Human brain membranes and the binding of [3H]GR65630 were carried out according to standardized methods. The highest density (Bmax ± 6 SD, fmol/mg protein) of [3H]GR65630 binding sites was found in area postrema (13.1 ± 9.7), followed at a statistically lower level, by nucleus tractus solitarius (6.7 ± 3.4), nervus vagus (5.5 ± 2.1), striatum (4.8 ± 2.4) with a progressive decrease in amygdala, olivar nuclei, hippocampus, olfactory bulbus and prefrontal cortex, and then by the other cortical areas and the cerebellum, where no binding was detected. These observations extend previous findings on the distribution of 5-HT3 receptors and confirm interspecies variations that might explain the heterogeneous properties of 5-HT3 receptors in different animals.

Serotoninserotonin receptors of type 3human brain postmortem[3H]GR65630 binding

Copyright information

© Plenum Publishing Corporation 2001