Plant Cell, Tissue and Organ Culture

, Volume 66, Issue 3, pp 189–197

Effectiveness of three micropropagation techniques to dissociate cytochimeras in Musa spp

  • Nicolas Roux
  • Jaroslav Dolezel
  • Rony Swennen
  • Fransisco J. Zapata-Arias

DOI: 10.1023/A:1010624005192

Cite this article as:
Roux, N., Dolezel, J., Swennen, R. et al. Plant Cell, Tissue and Organ Culture (2001) 66: 189. doi:10.1023/A:1010624005192


In vitro mutagenesis of multicellular meristems of Musa spp. leads to a high degree of chimerism. Repeated vegetative propagation must be carried out to dissociate chimeras but the minimum number of cycles required is unknown. In general, mutated cells are difficult to monitor but mutations which result in a change in genome number are an exception. We simulated this case by colchicine treatment, followed by flow cytometric analysis. Colchicine treatment induced ploidy chimerism (mixoploidy), and chimera dissociation was assessed using three different propagation systems (shoot-tip culture technique - ST, multi-apexing culture technique - MA and corm slice culture technique - CS). The average percentage of cytochimeras was reduced from 100% to 36% after three subcultures using shoot-tip culture, from 100% to 24% when propagating by the corm slice culture technique and from 100% to 8% after the same number of subcultures using the multi-apexing technique. All propagation systems failed to eliminate chimerism completely. Factors that may influence chimera dissociation in vitro are discussed.

chimeraflow cytometryin vitro tissue cultureMusa acuminatamixoploidypolyploidisation

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Nicolas Roux
    • 1
  • Jaroslav Dolezel
    • 2
  • Rony Swennen
    • 3
  • Fransisco J. Zapata-Arias
    • 4
  1. 1.FAO/IAEA Agriculture and Biotechnology Laboratory, International Atomic Energy Agency LaboratoriesSeibersdorfAustria
  2. 2.Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental BotanyOlomoucCzech Republic
  3. 3.Laboratory of Tropical Crop ImprovementKatholieke Universiteit LeuvenHeverleeBelgium
  4. 4.FAO/IAEA Agriculture and Biotechnology Laboratory, International Atomic Energy Agency LaboratoriesSeibersdorfAustria