Determination of Dopamine D1 receptors in the human uveo scleral tissue by light microscope autoradiography
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The aim of this paper is to clarify the distribution of Dopamine D1 (DA D1) receptors in the uveo-scleral tissue of human eyes with or without elevated intraocular pressure (IOP) and to study the relationships between DA D1 receptors and uveo-scleral tissue. Samples of human uveo-scleral tissue were taken from seven men undergoing eye surgery for a traumatic lesion of the anterior segment of the eye, without involvement of the iris-corneal angle and /or from eye donors. The subjects (in whom one eye bulb had been surgically enucleated) had been previously enrolled in our medical protocols because they suffered for increased IOP, while the eye donors (of both eye bulbs) had a normal IOP. Frozen sections from the uveo-scleral tissue were submitted to biochemical characterization and to morphological autoradiographic techniques for detection of DA-D1 receptors. [3H]-SCH-23390 was used as a ligand of Dopamine D1 receptors.[3H]-SCH 23390 was bound by sections of the human uveo-scleral tissue. The pharmacological profile of the binding was consistent with the labeling of D1 receptors. Light microscope analysis was used for localization of D1 receptors and revealed an accumulation of the radioligand in the human uveo-scleral tissue. In eyes with normal IOP there is a high reaction. The Bmax of radioligand decreases in the eyes with increased IOP.The possibility that dopaminergic receptors play a role in the controlling uveo-scleral tissue functions is suggested.
- Mancino RA, Cerulli L, Ricci A, Amenta F. Direct demonstration of dopamine D1-like receptor sites in the ciliary body of the rabbit eye by light microscope autoradiography. Naunyn-Schmiedberg Archives of Pharmacology 1992; 346: 644–648.
- Bill A. Uveo scleral drainage of aqueous humor: Physiology and Pharmacology. Progr Clin Biol Res 1989; 312: 417–427.
- Karnezis TA, Murphy MB, Weber RR, Nelson KS, Tripathi RC. Effects of selective dopamine-D1 receptor activation on intraocular pressure in man. Exp Eye Res 1988; 47: 689–697.
- Napoleone P, Cavallotti C, Strocchi P, Ricci A, Amenta F. Autoradiographic localization of Dopexamine binding sites and Dopexamine cyclic Adenosine Monophosphate generating system in the rat heart. Drug Dev Res 1992; 25: 97–106.
- Ferreira-de-Almeida JA, Pereira-Leite L, Cavallotti C, Ricci A, Amenta F. Pharmacological characterization and autoradiographic localization of dopamine D1 receptors in the human umbilical artery. Europ J Pharmacol 1993; 234: 209–214.
- Napoleone P, Cavallotti C, Ricci A, Amenta F. Dopexamine hydrochloride in the human kidney: Localization receptor binding and effect on 3- 5 Cyclic Adenosine Monophosphate generation. Nephron 1993; 65: 385–391.
- Escaf S, Cavallotti C, Ricci A, Vega JA, Amenta F. Dopamine D1 and D2 receptors in the human ureter and urinary bladder: a radioligand binding and autoradiographic study. Br J Urology 1994; 73: 473–479.
- Kobayashi Y, Ricci A, Amenta F, Cavallotti C. Localization of Dopamine receptors in the rabbit lung vasculature. J Vascul Res 1995; 32: 200–206.
- Amenta F, Ricci A. Autoradiographic localization of dopamine DA-D1 receptors in the rat renal vasculature using [3H]-SCH-23390 as a ligand. J Auton Pharmacol 1990;10: 373–383.
- Amenta F, Ricci A, Vega JA. Autoradiographic localization of dopamine receptors in the rat cerebral blood vessels. Eur J Pharmacol 1991; 192: 123–132.
- Amenta F. Biochemistry and autoradiography of peripheral dopamine receptors. In: Amenta F, ed. Peripheral Dopamine Pathophysiology. Boca Raton, USA: CRC Press, 1990b; 39–51.
- Cramer H. Mathematical methods of statistics. Princeton, USA 1986.
- Cheng YC, Prussoff WH. Relationship between inhibition constant (ki) and the concentration of inhibitor which causes 50 per cent of inhibition (IC-50) of an enzymatic reaction. Biochem Pharmacol 1973; 22: 3099–3108.
- Ersenhart H, ed. Selected techniques of Statistical analysis for Scientific Research. New York 1977.
- Brodde OE. Affinity of dopamine agonists and antagonists for peripheral dopamine receptors: comparison of functional studies with radioligand binding studies. In: Hieble JP, ed. Cardiovascular function of peripheral dopamine receptors. Dekker, New York-Basel: 1989; 27–72.
- Green K, Elijah D. Drug effects on aqueous humor formation and pseudo-facility in normal rabbit eye. Exp Eye Res 1979; 33: 239–245.
- Guyton AC. Textbook of Human Physiology. Philadelphia: Saunders, 1971.
- Prunte C, Nuttli I, Markstein R, Kohler C. Effects of dopamine D1 and D2 receptors on intraocular pressure in conscious rabbit. J Neural Transm 1997; 104: 111–123.
- Potter DE, Burke JA, Chang FW. Alteration in ocular function induced by phenylethylamine analogues of dopamine. Curr Eye Res 1984; 3: 851–859.
- Piltz JR, Stone RA, Boike S, Everitt DE, Shusterman NH, Andet P, Zariffa N, Jorkasky DK. Fenoldopam, a selective Dopamine D1 receptor agonist, raises intraocular pressure in males with normal intraocular pressure. J Ocular Pharmacol Ther 1998; 14: 203–216.
- Prunte C, Flammer J. The novel dopamine D1 antagonist and D2 agonist, SDZ GLC-756, lowers intraocular pressure in healthy human volunteers and in patients with glaucoma. Ophthalmology 1995; 102: 1291–1297.
- Prunte C, Nuttli I, Markstein R. Topical SDZ GLC-756, a novel dopamine D1 antagonist and D2 agonist, lowers intraocular pressure in conscious rabbits. Jpn J Ophthalmol 1996; 40: 167–173.
- Determination of Dopamine D1 receptors in the human uveo scleral tissue by light microscope autoradiography
Volume 23, Issue 3 , pp 171-179
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- uveo-scleral tissue
- intraocular pressure
- dopamine D1 receptors
- human eye
- irido-corneal angle
- Industry Sectors
- Author Affiliations
- 1. 3rd Chair of Human Anatomy, University `La Sapienza', Rome, Italy
- 2. Eye Clinic, University `La Sapienza', Rome, Italy
- 3. Department of Neurosciences, University `La Sapienza', Rome, Italy