Plant Molecular Biology

, Volume 47, Issue 1, pp 295–310

Enabling technologies for manipulating multiple genes on complex pathways

  • Claire Halpin
  • Abdellah Barakate
  • Barak M. Askari
  • James C. Abbott
  • Martin D. Ryan
Article

DOI: 10.1023/A:1010604120234

Cite this article as:
Halpin, C., Barakate, A., Askari, B.M. et al. Plant Mol Biol (2001) 47: 295. doi:10.1023/A:1010604120234

Abstract

Many complex biochemical pathways in plants have now been manipulated genetically, usually by suppression or over-expression of single genes. Further exploitation of the potential for plant genetic manipulation, both as a research tool and as a vehicle for plant biotechnology, will require the co-ordinate manipulation of multiple genes on a pathway. This goal is currently very difficult to achieve. A number of approaches have been taken to combine or `pyramid' transgenes in one plant and have met with varying degrees of success. These approaches include sexual crossing, re-transformation, co-transformation and the use of linked transgenes. Novel, alternative `enabling' technologies are also being developed that aim to use single transgenes to manipulate the expression of multiple genes. A chimeric transgene with linked partial gene sequences placed under the control of a single promoter can be used to co-ordinately suppress numerous plant endogenous genes. Constructs modelled on viral polyproteins can be used to simultaneously introduce multiple protein-coding genes into plant cells. In the course of our work on the lignin biosynthetic pathway, we have tested both conventional and novel methods for achieving co-ordinate suppression or over-expression of up to three plant lignin genes. In this article we review the literature concerning the manipulation of multiple genes in plants. We also report on our own experiences and results using different methods to perform directed manipulation of lignin biosynthesis in tobacco.

co-ordinate expressionligninmultiple transgenespolyproteinspyramidingtransgene silencing

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Claire Halpin
    • 1
  • Abdellah Barakate
    • 1
  • Barak M. Askari
  • James C. Abbott
    • 1
  • Martin D. Ryan
    • 2
  1. 1.Division of Environmental and Applied Biology, School of Life SciencesUniversity of DundeeDundeeUK
  2. 2.Division of Biomedical Science, School of BiologyUniversity of St. AndrewsFifeUK