Article

Biotechnology Letters

, Volume 23, Issue 10, pp 767-770

First online:

High-density culture of recombinant Chinese hamster ovary cells producing prothrombin in protein-free medium

  • Zhaolie ChenAffiliated withDepartment of Cell Engineering, Institute of Biotechnology
  • , Dirk LütkemeyerAffiliated withInstitute of Cell Culture Technology, University of Bielefeld
  • , Kai IdingAffiliated withInstitute of Cell Culture Technology, University of Bielefeld
  • , Jürgen LehmannAffiliated withInstitute of Cell Culture Technology, University of Bielefeld

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Abstract

A recombinant CHO cell line, CHO2DS, was immobilized on porous microcarrier Cytopore 1 and cultivated in 1 l modified Super-spinner and 2 l stirred tank bioreactor with the perfusion of a low-cost chemically defined protein-free medium DF6S. CHO2DS cells could enter into the inner space and grew both in the inner space and on the surface of Cytopore 1 in DF6S and produced prothrombin at 22 mg l−1 after 10 days. From a seeding density of 5.7 × 105 cells ml−1, the highest viable cell density of CHO2DS was 1.12 × 107 cells ml−1.

high-density cultivation protein-free medium prothrombin recombinant CHO cell