Plant and Soil

, Volume 232, Issue 1, pp 97–108

Soil and rhizosphere as habitats for Pseudomonas inoculants: new knowledge on distribution, activity and physiological state derived from micro-scale and single-cell studies

  • Jan Sørensen
  • Linda Elise Jensen
  • Ole Nybroe
Article

DOI: 10.1023/A:1010338103982

Cite this article as:
Sørensen, J., Jensen, L.E. & Nybroe, O. Plant and Soil (2001) 232: 97. doi:10.1023/A:1010338103982

Abstract

Pseudomonas spp. comprise an important group of bacteria used for biological control of microfungi in the plant rhizosphere. Successful performance of microbial inoculants requires both establishment, proliferation and activity under in situ conditions. To identify the factors controlling fate and performance of the inoculants, small-scale analyses are needed due to the heterogeneity characterizing the complex soil and rhizosphere environments. Direct staining techniques and advanced microscopy have provided the first detailed single-cell images of root colonization by these bacteria using fluorescent antibodies, fluorescent in situ hybridization and marker gene technology. These tracking methods have, in conjunction with activity assays, provided high-resolution data on the metabolic activity and growth of the inoculants. Finally, Pseudomonas reporter bacteria constructed to sense phosphorus, nitrogen, iron, and oxygen limitations have provided new insight into the significance of growth-limiting factors in the soil and along the root. The present work reviews the current knowledge on Pseudomonas inoculants in soil and rhizosphere based on these modern techniques. Finally, some perspectives for future studies are discussed.

Pseudomonas rhizosphere root colonization N-limitation reporter bacteria 

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Jan Sørensen
    • 1
  • Linda Elise Jensen
    • 1
  • Ole Nybroe
    • 1
  1. 1.Section of Genetics and Microbiology, Department of EcologyRoyal Veterinary and Agricultural UniversityFrederiksberg CDenmark