Biotechnology Letters

, Volume 23, Issue 7, pp 551–554

Phytase production by the yeast, Pichia anomala

  • Ashima Vohra
  • T. Satyanarayana

DOI: 10.1023/A:1010314114053

Cite this article as:
Vohra, A. & Satyanarayana, T. Biotechnology Letters (2001) 23: 551. doi:10.1023/A:1010314114053


Pichia anomala, isolated from dried flower buds of Woodfordia fruticosa, produced a high activity of an intracellular phytase, at 68 U per g dry biomass, when grown at 20 °C for 24 h in a medium containing glucose (40 g l−1) and beef extract (10 g l−1) supplemented with Fe2+ (0.15 mM). Partially purified phytase was optimally active at 60 °C and pH 4 with a half life of 7 days at 60 °C. It retained 85% of its activity at 80 °C for 15 min. The enzyme is suitable for supplementing animal feeds to improve the availability of phosphate from phytate.

phytase Pichia anomala yeast 

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Ashima Vohra
    • 1
  • T. Satyanarayana
    • 1
  1. 1.Department of MicrobiologyUniversity of Delhi, South CampusNew Delhi 110 021India