Identification of a Human LNX Protein Containing Multiple PDZ Domains
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Recently PDZ5 domains have been recognized as crucial organizers of protein complexes at the plasma membrane. Here we report the cloning of a novel 3.7-kb cDNA LNX from a human fetal brain cDNA library. The deduced amino acid sequence shares about 88% identity with the p70 form of Lnx, which is the mouse ligand of Numb protein X. Motif analysis reveals five protein-interaction modules conservative to those in the Lnx protein, including four PDZ domains and a NPXY motif for binding of the Numb PTB domain. Northern blot analysis shows that the length of the transcript in brain is distinct from those in other tissues. By radiation hybrid mapping, we localize the LNX gene to human chromosome 4q12 between marker D4S1577 and marker D4S1594. We predict that LNX may be important for the organization of signaling complexes in Numb-involved pathways or pathways regulated by other PTB-containing proteins.
- Burack, W. R., and Shaw, A. S. (2000). Signal transduction: Hanging on a scaffold. Curr. Opin. Cell Biol. 12: 211.
- Cabral, J. H. M., Petosa, C., Sutcliffe, M. J., Raza, S., Byron, O., Poy, F., Marfatia, S. M., Chishti, A. H., and Liddington, R. C. (1996). Crystal structure of a PDZ domain. Nature 382: 649.
- Dho, S. E., Jacob, S., Wolting, C. D., French, M. B., Rohrscheinder, L. R., and McGlade, C. J. (1998). The mammalian numb phosphotyrosine-binding domain-Characterization of binding specificity and identification of a novel PDZ domain-containing Numb binding protein, LNX. J. Biol. Chem. 273(15): 9179.
- Dho, S. E., French, M. B., Woods, S. A., and McGlade, C. J. (1999). Characterization of four mammalian numb protein isoforms. J. Biol. Chem. 274(46): 33097.
- Doyle, D. A., Lee, A., Lewis, J., Kim, E., Sheng, M., and MacKinnon, R. (1996). Crystal structure of a complexed and peptide-free membrane protein-binding domain: Molecular basis of peptide recognition by PDZ. Cell 85: 1067.
- Fanning, A. S., and Anderson, J. M. (1996). Protein-protein interactions: PDZ domain networks. Curr. Biol. 6: 1355.
- Fanning, A. S., and Anderson, J. M. (1999). PDZ domains: Fundamental building blocks in the organization of protein complexes at the plasma membrane. J. Clin. Invest. 103(6): 767.
- Gokhale, R. S., and Khosla, C. (2000). Role of linkers in communication between protein modules. Curr. Opin. Chem. Biol. 4: 22.
- Harrison, S. C. (1996). Peptide-surface association: The case of PDZ and PTB domains. Cell 86: 341.
- Kornau, H. C., Seeburg, P. H., and Kennedey, M. B. (1997). Interaction of ion channels and receptors with PDZ domain proteins. Curr. Opin. Neurobiol. 7: 368.
- Matsuzaki, F. (2000). Asymmetric division of Drosophila neural stem cells:Abasis for neural diversity. Curr. Opin. Neurobiol. 10: 38.
- Pawson, T., and Scott, J. D. (1997). Signaling through scaffold, anchoring and adapter proteins. Science 278: 2075. CrossRef
- Ponting, C. P., Phillips, C., Davies, K. E., and Blake, D. J. (1997). PDZ domains: Targeting molecules to sub-membranous sites. Bioessays 19: 469.
- Scott, K., and Zuker, C. S. (1998). Assembly of the Drosophila phototransduction cascade into a signaling complex shapes elementary responses. Nature 395: 805.
- Shawn Xu, X. Z., Choudhury, A., Li, X., and Montell, C. (1998). Coordination of an array of signaling proteins through homo-and heteromeric interactions between PDZ domains and target proteins. J. Cell Biol. 142(2): 545.
- Songyang, Z., Fanning, A. S., Fu, C., Xu, J., Marfatia, S. M., Chishti, A. H., Crompton, A, Chan, A. C., Anderson, J. M., and Cantley, L. C. (1997). Recognition of unique carboxyl-terminal motifs by distinct PDZ domains. Science 275: 73.
- Spana, E. P., and Doe, C. Q. (1996). Numb antagonizes notch signaling to specify sibling neuron cell fates. Neuron 17: 21.
- Yeaman, C., Grindstaff, K. K., Hansen, M. D. H., and Nelson, W. J. (1999). Cell polarity: Versatile scaffolds keep things in place. Curr. Biol. 9: R515.
- Identification of a Human LNX Protein Containing Multiple PDZ Domains
Volume 39, Issue 3-4 , pp 117-126
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers-Plenum Publishers
- Additional Links
- ligand of Numb protein X
- signal transduction
- asymmetric cell division