Abstract
The collection of time-series of periphyton biomass is a difficult task due to the destructive nature of the standard methods. A non-destructive method based on photography and digitalization, for the estimation of Chla of periphyton colonizing artificial substrata is presented. The standard spectrophotometric method was used to obtain a calibration curve. The relative errors of the proposed method were similar to those of other published methods. The photographic method should be used when a large quantity of samples from the same community is needed and a high precision on the individual measurement is not required.
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References
Aminot A (1983) Dosage de la chlorophylle et des phéopigments par spectrophotométrie. In: Aminot A and Chausspied N (eds), Manuel des analyses chimiques en milieu marin. (pp. 177–189) Centre National pour l'exploitation des Océans, Québec
Becker G, Holfeld H, Hasselrot AT, Fiebig DM and Menzler DA (1997) Use of a microscope photometer to analyze in vivo fluorescence intensity of epilithic microalgae grown on artificial sustrata. Appl Environ Microbiol 63: 1318–1325
Hänninen O, Ruuskanen J and Oksanen J (1993) A method for facilitating the use of algae growing on tree trunks ass bioindicators of air quality. Environ Monit Assessment 28: 215–220
Lawrence WH, Itsweire EC and Esaias WE (1994) Estimates of Phytoplankton Biomass in the Chesapeake Bay from Aircraft Remote Sensing of Chlorophyll Concentrations, 1989–92. Remote Sens Environ 49: 41–56
Lowe RL, Guckert JB, Belanger SE, Davidson DH and Johnson DW (1996) An evaluation of periphyton community structure and function on tile and cobble substrata in experimental stream mesocosms. Hydrobiologia 328: 135–146
Gitelson AA, Laorawat S, Galya PK and Vonshak A (1995) Optical properties of dense algal cultures outdoors and their application to remote estimation of biomass and pigment concentration in Spirulina platensis (Cyanobacteria). J Phycol 31: 828–834
Morin A and Cattaneo A (1992) Factors affecting sampling variability of freshwater periphyton and the power of periphyton studies. Can J Fish Aquat Sci 49: 1695–1703
Nusch EA (1980) Comparison of different methods for chlorophyll and phaeopigment determination. Arch Hydrobiol Brih Ergebn Limnol 14: 14–36
Peñuelas J, Gamon JA, Griffin KL and Field CB (1993) Assesing community type, plant biomass, pigment composition, and photosynthetic efficiency of aquatic vegetation from spectral reflectance. Remote Sens Environ 46: 110–118
Sartory DP and Grobbelar JU (1984) Extraction of chlorophyll a from freshwater phytoplankton for spectrophotometric analysis. Hydrobiologia 114: 177–187
Thieberger Y, Kizner Z, Achituv Y and Dubinsky Z (1995) A novel, nondestructive bioassay for assessing areal chlorophyll a in hermatypic cnidarians. Limnol Oceanogr 40: 1166–1173
Wetzel RG (1964) A comparative study of the primary productivity of higher aquatic plants, periphyton, and phytoplankton in a large, shallow lake. Int Revue Ges Hydrobiol 49: 1–61
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Saravia, L.A., Giorgi, A. & Momo, F.R. A photographic method for estimating chlorophyll in periphyton on artificial substrata. Aquatic Ecology 33, 325–330 (1999). https://doi.org/10.1023/A:1009934626188
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DOI: https://doi.org/10.1023/A:1009934626188