Methods in Cell Science

, Volume 22, Issue 1, pp 51-61

First online:

Methods for animal satellite cell culture under a variety of conditions

  • Nycole M. Burton
  • , Janet/fnms>  Vierck
  • , Lyssa Krabbenhoft
  • , Katherine Bryne
  • , Michael V. Dodson

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


Primary and clonal culture systems have been devised and refined for animal-derived satellite cells. Satellite cell (SC) culture development includes efficient cell isolation techniques, establishment of effective plating and growth conditions, formulation of media requirements and thorough evaluation of experimental limitations. As the field of muscle cell culture has expanded, the number of animal species from which satellite cells have been isolated has increased. The focus of this paper is to compare and contrast SC culture conditions presently used by a variety of researchers and to introduce a new source of SC from wapiti (elk).

Cell culture comparisons Satellite cells Wapiti