Molecular Breeding

, Volume 4, Issue 4, pp 277–289

Overview of DNA chip technology

  • Bertrand Lemieux
  • Asaph Aharoni
  • Mark Schena
Article

DOI: 10.1023/A:1009654300686

Cite this article as:
Lemieux, B., Aharoni, A. & Schena, M. Molecular Breeding (1998) 4: 277. doi:10.1023/A:1009654300686

Abstract

DNA chip technology utilizes microscopic arrays (microarrays) of molecules immobilized on solid surfaces for biochemical analysis. Microarrays can be used for expression analysis, polymorphism detection, DNA resequencing, and genotyping on a genomic scale. Advanced arraying technologies such as photolithograpy, micro-spotting and ink jetting, coupled with sophisticated fluorescence detection systems and bioinformatics, permit molecular data gathering at an unprecedented rate. Microarray-based characterization of plant genomes has the potential to revolutionize plant breeding and agricultural biotechnology. This review provides an overview of DNA chip technology, focusing on manufacturing approaches and biological applications.

genomicsgenotypingmicroarraysmutation detectiontranscript monitoring

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Bertrand Lemieux
    • 1
  • Asaph Aharoni
    • 2
  • Mark Schena
    • 3
  1. 1.Department of Plant and Soil SciencesUniversity of DelawareNewarkUSA
  2. 2.Department of Cell BiologyDLO – Center for Plant Breeding and Reproduction ResearchWageningenThe Netherlands
  3. 3.Department of Biochemistry, Beckman CenterStanford University School of MedicineStanfordUSA