Article

Molecular Breeding

, Volume 6, Issue 1, pp 47-53

First online:

Expression of correctly processed human growth hormone in seeds of transgenic tobacco plants

  • Adilson LeiteAffiliated withDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo
  • , Edson L. KemperAffiliated withDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo
  • , Márcio J. da SilvaAffiliated withDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo
  • , Augusto D. LuchessiAffiliated withDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo
  • , Rodrigo M.P. SilotoAffiliated withDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo
  • , Eric D. BonaccorsiAffiliated withDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo
  • , Hamza F. El-DorryAffiliated withDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo
  • , Paulo ArrudaAffiliated withDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo

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Abstract

Human growth hormone was expressed in transgenic tobacco seeds using the monocot tissue-specific promoter from sorghum γ-kafirin seed storage protein gene. During tobacco seed ripening, the expressed hormone was directed to the endoplasmic reticulum by a signal peptide from a Coix prolamin and was secreted into the apoplastic space, where it accounted for 0.16% of total soluble seed protein. The expressed hormone has the same amino acid sequence and receptor-binding properties as the native mature hormone.

seed-specific expression human growth hormone plant farming heterologous protein transgenic plants