Separate and variably shaped chromosome arm domains are disclosed by chromosome arm painting in human cell nuclei
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- Dietzel, S., Jauch, A., Kienle, D. et al. Chromosome Res (1998) 6: 25. doi:10.1023/A:1009262223693
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Fluorescence in situ hybridization (FISH) with microdissection probes from human chromosomes 3 and 6 was applied to visualize arm and subregional band domains in human amniotic fluid cell nuclei. Confocal laser scanning microscopy and quantitative three-dimensional image analysis showed a pronounced variability of p- and q-arm domain arrangements and shapes. Apparent intermingling of neighbouring arm domains was limited to the domain surface. Three-dimensional distance measurements with pter and qter probes supported a high variability of chromosome territory folding.