, Volume 13, Issue 1, pp 65–72

A gene of the major facilitator superfamily encodes a transporter for enterobactin (Enb1p) in Saccharomyces cerevisiae

  • Petra Heymann
  • Joachim F. Ernst
  • Günther Winkelmann

DOI: 10.1023/A:1009250017785

Cite this article as:
Heymann, P., Ernst, J.F. & Winkelmann, G. Biometals (2000) 13: 65. doi:10.1023/A:1009250017785


While in fungi iron transport via hydroxamate siderophores has been amply proven, iron transport via enterobactin is largely unknown. Enterobactin is a catecholate-type siderophore produced by several enterobacterial genera grown in severe iron deprivation. By using the KanMX disruption module in vector pUG6 in a fet3Δ background of Saccharomyces cerevisiae we were able to disrupt the gene YOL158c Sce of the major facilitator super family (MFS) which has been previously described as a gene encoding a membrane transporter of unknown function. Contrary to the parental strain, the disruptant was unable to utilize ferric enterobactin in growth promotion tests and in transport assays using 55Fe-enterobactin. All other siderophore transport properties remained unaffected. The results are evidence that in S. cerevisiae the YOL158c Sce gene of the major facilitator super family, now designated ENB1, encodes a transporter protein (Enb1p), which specifically recognizes and transports enterobactin.

major facilitator superfamilyiron transportsiderophoresenterobactinSaccharomyces cerevisiae

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Petra Heymann
  • Joachim F. Ernst
  • Günther Winkelmann

There are no affiliations available