Continuous solvent production by Clostridium beijerinckii BA101 immobilized by adsorption onto brick

  • N. Qureshi
  • J. Schripsema
  • J. Lienhardt
  • H.P. Blaschek
Article

DOI: 10.1023/A:1008984509404

Cite this article as:
Qureshi, N., Schripsema, J., Lienhardt, J. et al. World Journal of Microbiology and Biotechnology (2000) 16: 377. doi:10.1023/A:1008984509404

Abstract

The performance of a continuous bioreactor containing Clostridium beijerinckii BA101 adsorbed onto clay brick was examined for the fermentation of acetone, butanol, and ethanol (ABE). Dilution rates from 0.3 to 2.5 h−1 were investigated with the highest solvent productivity of 15.8 g l−1 h−1 being obtained at 2.0 h−1. The solvent yield at this dilution rate was found to be 0.38 g g−1 and total solvent concentration was 7.9 g l−1. The solvent yield was maximum at 0.45 at a dilution rate of 0.3 h−1. The maximum solvent productivity obtained was found to be 2.5 times greater than most other immobilized continuous and cell recycle systems previously reported for ABE fermentation. A higher dilution rate (above 2.0 h−1) resulted in acid production rather than solvent production. This reactor was found to be stable for over 550 h. Scanning electron micrographs (SEM) demonstrated that a large amount of C. beijerinckii cells were adsorbed onto the brick support.

Acetone–butanol–ethanol (ABE) fermentationcell immobilizationClostridium beijerinckii BA101continuous reactor

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • N. Qureshi
    • 1
  • J. Schripsema
    • 1
  • J. Lienhardt
    • 1
  • H.P. Blaschek
    • 1
  1. 1.Department of Food Science & Human Nutrition, Biotechnology & Bioengineering GroupUniversity of IllinoisUrbanaUSA