Article

Journal of Materials Science: Materials in Medicine

, Volume 10, Issue 3, pp 129-134

First online:

Synthetic collagen fibers coated with a synthetic peptide containing the YIGSR sequence of laminin to promote peripheral nerve regeneration in vivo

  • S. ItohAffiliated withDepartment of Orthopaedic Surgery, Tokyo Medical and Dental University
  • , K. TakakudaAffiliated withDivision of Applied Mechanics Institute for Medical and Dental Engineering, Tokyo Medical and Dental University
  • , H. SamejimaAffiliated withDepartment of Orthopaedic Surgery, Tokyo Medical and Dental University
  • , T. OhtaAffiliated withDepartment of Orthopaedic Surgery, Tokyo Medical and Dental University
  • , K. ShinomiyaAffiliated withDepartment of Orthopaedic Surgery, Tokyo Medical and Dental University
  • , S. IchinoseAffiliated withCenter for Electron Microscopy, Tokyo Medical and Dental University

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Abstract

The usefulness of collagen fibers and the YIGSR sequence (Tyr-lle-Gly-Ser-Arg) of laminin for nerve regeneration were examined in vivo. Type I collagen gel (G-group), Type I collagen fibers (F-group), Type I collagen fibers coated with laminin (L-group) or the YIGSR sequence (Y-group) were packed into silicone tubes, 15 mm long, and transplanted to the sciatic nerves of Wistar rats. Empty silicone tubes were used as the control. The animals were sacrificed 8 weeks after transplantation. Bridging of the nerve was confirmed in the F-(7/12), Y-(7/10) and L-group (6/10), but no bridging was observed in any of the animals of the G- and control group. Nerve regeneration among the space of collagen fibers was observed, and it was suggested that fibroblasts infiltrated the gap in the substance of the degenerated collagen fibers were followed by Schwann cells on the basis of immunocytochemistry. The number of myelinated axons per regenerated tissue in the tube (density), and total area of myelinated axons per measured regenerated tissue in the tube (% axon area) in each the L- and Y-group were significantly higher than that in the F-group (P < 0.05). These results suggest the possibility of obtaining adequate nerve regeneration with new artificial materials only. © 1999 Kluwer Academic Publishers