World Journal of Microbiology and Biotechnology

, Volume 16, Issue 8, pp 799–803

Production of ligninolytic enzymes by Fusarium solani strains isolated from different substrata

Authors

  • Mario Carlos Nazareno Saparrat
    • Instituto de Botánica Spegazzini, Facultad de Ciencias Naturales y MuseoUniversidad Nacional de La Plata
  • María Jesús Martínez
    • Unidad de Microbiología Aplicada, Centro de Investigaciones BiológicasConsejo Superior de Investigaciones Científicas
  • Horacio Alfio Tournier
    • Cátedra de Farmacología, Facultad de Ciencias MédicasUniversidad Nacional de La Plata
  • Marta Noemí Cabello
    • Instituto de Botánica Spegazzini, Facultad de Ciencias Naturales y MuseoUniversidad Nacional de La Plata
  • Angélica Margarita Arambarri
    • Instituto de Botánica Spegazzini, Facultad de Ciencias Naturales y MuseoUniversidad Nacional de La Plata
Article

DOI: 10.1023/A:1008923804859

Cite this article as:
Saparrat, M.C.N., Martínez, M.J., Tournier, H.A. et al. World Journal of Microbiology and Biotechnology (2000) 16: 799. doi:10.1023/A:1008923804859

Abstract

A comparative study on the extracellular ligninolytic enzymatic activity of five strains of Fusarium solani in a carbon-limited medium under shaking, revealed a differential production of these enzymes. Aryl alcohol oxidase (AAO) activity was observed only in the supernatant of strain CLPS no. 568 with levels higher than 57 mU ml−1. Free extracellular laccase activity was detected in strains CLPS nos. 493, 568 and 570, strain no. 568 being the one which showed the highest activity (over 8.6 mU ml−1). Free extracellular lignin peroxidase (LiP) activity was not detected in any isolate tested, whereas low levels of manganese-dependent peroxidase (MnP) and manganese-independent peroxidase (MIP) activities were detected in certain isolates used. The AAO activity of F. solani on primary α-alcohols such as veratryl alcohol, is reported for the first time; this enzyme activity is hydrogen-peroxide independent. This is also the first report for extracellular MnP and MIP activities of F. solani.

Aryl-alcohol oxidaseextracellular enzymesFusariumlaccaselignin peroxidasemanganese-dependent peroxidasemanganese-independent peroxidase

Copyright information

© Kluwer Academic Publishers 2000