Article

Biotechnology Techniques

, Volume 13, Issue 8, pp 523-528

A probing feeding strategy for Escherichia coli cultures

  • Mats ÅkessonAffiliated withDepartment of Automatic Control, Lund Institute of Technology
  • , Per HaganderAffiliated withDepartment of Automatic Control, Lund Institute of Technology
  • , Jan Peter AxelssonAffiliated withPharmacia & Upjohn, Process R&D

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Abstract

A strain-independent feeding strategy for fed-batch cultures of Escherichia coli is presented. By superimposing short pulses in the glucose feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation. The feasibility of the strategy is demonstrated by both simulation and experiments.

acetate dissolved oxygen Escherichia coli feedback control glucose feeding