A probing feeding strategy for Escherichia coli cultures
- Cite this article as:
- Åkesson, M., Hagander, P. & Axelsson, J.P. Biotechnology Techniques (1999) 13: 523. doi:10.1023/A:1008906517033
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A strain-independent feeding strategy for fed-batch cultures of Escherichia coli is presented. By superimposing short pulses in the glucose feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation. The feasibility of the strategy is demonstrated by both simulation and experiments.