Biotechnology Techniques

, Volume 13, Issue 8, pp 523–528

A probing feeding strategy for Escherichia coli cultures


  • Mats Åkesson
    • Department of Automatic ControlLund Institute of Technology
  • Per Hagander
    • Department of Automatic ControlLund Institute of Technology
  • Jan Peter Axelsson
    • Pharmacia & Upjohn, Process R&D

DOI: 10.1023/A:1008906517033

Cite this article as:
Åkesson, M., Hagander, P. & Axelsson, J.P. Biotechnology Techniques (1999) 13: 523. doi:10.1023/A:1008906517033


A strain-independent feeding strategy for fed-batch cultures of Escherichia coli is presented. By superimposing short pulses in the glucose feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation. The feasibility of the strategy is demonstrated by both simulation and experiments.

acetatedissolved oxygenEscherichia colifeedback controlglucose feeding

Copyright information

© Kluwer Academic Publishers 1999