Biotechnology Techniques

, Volume 12, Issue 11, pp 829–832

Genetic transformation of Pseudomonas oleovorans by electroporation

  • Daniel K.Y. Solaiman
Article

DOI: 10.1023/A:1008881121726

Cite this article as:
Solaiman, D.K. Biotechnology Techniques (1998) 12: 829. doi:10.1023/A:1008881121726

Abstract

An electroporation procedure for the transformation of Pseudomonas oleovorans was developed using a model plasmid, pCN51. The optimal electrotransformation was achieved with cells harvested at 45 to 60 min of growth and concentrated to a cell density of 5 OD600nm, plasmid concentration of 6 μg per 100 μl of cell suspension, and a 0.1-cm gap-width cuvette. Electroporation was performed at the settings of 250 ω, 25μF and 2.5 kV. Transformation yields in the order of 103 colony-forming-unit per electroporation sample were obtained. This is a first report of the electroporation of the commercially valuable bacterium Ps. oleovorans. © Rapid Science Ltd. 1998

Pseudomonas oleovoranselectroporationtransformationpoly(β-hydroxyalkanoate)alkane

Copyright information

© Chapman and Hall 1998

Authors and Affiliations

  • Daniel K.Y. Solaiman
    • 1
  1. 1.U.S. Department of Agriculture, ARS, NAAEastern Regional Research CenterEast Mermaid Lane WyndmoorUSA