, Volume 12, Issue 10, pp 743-746

Integrative and autonomously replicating vectors for analysis of promoters in Corynebacterium glutamicum

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Autonomously replicating shuttle (Escherichia coli – Corynebacterium glutamicum) promoter-probe vector pET2 and integrative promoter-probe vector pRIM2 for C. glutamicum were constructed. Transcriptional fusions of promoter-carrying fragments to the promoterless chloramphenicol acetyltransferase gene (cat) carried by the vectors can be used to determine position, strength and regulation of the respective promoters in multicopy system (pET2) and in single-copy system (pRIM2) and to perform deletion and mutation studies of the promoters. Utility of the vectors was shown on three C. glutamicum promoters. © Rapid Science Ltd. 1998