Biotechnology Techniques

, Volume 12, Issue 7, pp 553–556

Rapid method for the determination of lipid from the green alga Botryococcus braunii

  • Seog June Lee
  • Byung-Dae Yoon
  • Hee-Mock Oh
Article

DOI: 10.1023/A:1008811716448

Cite this article as:
Lee, S.J., Yoon, BD. & Oh, HM. Biotechnology Techniques (1998) 12: 553. doi:10.1023/A:1008811716448
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Abstract

Of various methods for lipid recovery in Botryococcus braunii UTEX 572, the most effective method was disruption of the cells with a bead-beater followed by extraction with chloroform/methanol (2:1, v/v). This gave a lipid content of 28.6% of dry wt. There was a significant relationship between in vivo fluorescence of cells stained with Nile Red and lipid content in B. braunii determined gravimetrically (r2 = 0.997). This suggested that the Nile Red staining as a rapid method was as good as the gravimetric method commonly used for lipid determination which requires toxic solvents and considerable time-consuming manipulations. © Rapid Science Ltd. 1998

Copyright information

© Chapman and Hall 1998

Authors and Affiliations

  • Seog June Lee
  • Byung-Dae Yoon
  • Hee-Mock Oh

There are no affiliations available