Biotechnology Techniques

, Volume 13, Issue 1, pp 23–27

Design of a thermostable cell adhesion protein

  • Eiry Kobatake
  • Koji Onoda
  • Yasuko Yanagida
  • Tetsuya Haruyama
  • Masuo Aizawa
Article

DOI: 10.1023/A:1008809415499

Cite this article as:
Kobatake, E., Onoda, K., Yanagida, Y. et al. Biotechnology Techniques (1999) 13: 23. doi:10.1023/A:1008809415499

Abstract

The design principle of a thermostable functional protein has been proposed by demonstrating genetic engineering synthesis of a thermostable cell adhesion protein. The cell adhesive peptide sequence, Arg-Gly-Asp (RGD), was incorporated into the elastin-based polyhexapeptide, whose repeating unit is Ala-Pro-Gly-Val-Gly-Val (APGVGV). The resulting protein possesses cell adhesion activity approximately 80% of fibronectin. After autoclaving at 120 °C for 20 min, the protein retained over 90% of cell adhesion activity, while the activity of autoclaved fibronectin decreased to 50%.

cell adhesion elastin polyhexapeptide RGD sequence thermostavle protein 

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Eiry Kobatake
    • 1
  • Koji Onoda
    • 1
  • Yasuko Yanagida
    • 1
  • Tetsuya Haruyama
    • 1
  • Masuo Aizawa
    • 1
  1. 1.Department of Bioengineering, Faculty of Bioscience and BiotechnologyTokyo Institute of Technology, NagatsutaMidori-ku, YokohamaJapan