European Journal of Plant Pathology

, Volume 103, Issue 4, pp 363–371

Characterisation of the European pathogen population of Magnaporthe grisea by DNA fingerprinting and pathotype analysis

  • E. Roumen
  • M. Levy
  • J.L. Notteghem
Article

DOI: 10.1023/A:1008697728788

Cite this article as:
Roumen, E., Levy, M. & Notteghem, J. European Journal of Plant Pathology (1997) 103: 363. doi:10.1023/A:1008697728788

Abstract

The genetic variability among 41 isolates of the blast pathogen (Magnaporthe grisea) from five European rice growing countries was studied. The genealogy of the isolates was investigated by DNA fingerprinting and the results compared to the degree of similarity for (a)virulence factors. Fingerprinting grouped the isolates into five discrete lineages, that typically showed less than 65% band similarity. Within each lineage, two or more haplotypes were detected with a band similarity of 80% or higher. Each lineage showed a characteristic virulence pattern. All isolates of lineage ‘E5’ belonged to the same pathotype. The other lineages were composed of clusters of closely related pathotypes that showed variation for virulence to cultivars with certain known resistance genes, while remaining invariably (a)virulent to others. In most cases, lineage classification of an isolate could be easily inferred by its pathotype. Certain resistance genes and certain lineage-excluding resistance gene combinations appear to provide protection against all of the virulence factors sampled.

blastriceMagnaporthe griseaPyriculariavirulencegenetic variability

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • E. Roumen
    • 1
  • M. Levy
    • 2
  • J.L. Notteghem
    • 3
  1. 1.BIOTEC, NSTDABangkokThailand
  2. 2.Department of Biological SciencesPurdue UniversityWest LafayetteUSA
  3. 3.Unité de Recherche Phytopathologie et Malherbologie, CIRAD-CAMontpellier CX1France