Intraspecific and interspecific variation in Setaria revealed by RAPD analysis
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Random amplified polymorphic DNA (RAPD) analysis was applied to foxtail millet (Setaria italica) and other Setaria species in order to assess the degree of polymorphism among and within the species and to investigate their genetic relationships. Twenty accessions of foxtail millet, five of green foxtail (S. viridis), five of yellow foxtail (S. glauca), one each of S. germanica, S. verticilliformis, S. pallidifusca, S. macrostachya, S. sphacelata and S. faberi, and one unidentified, were evaluated for variability using a set of 19 random 10-mer primers. A total of 148 markers were scored for all the accessions. Wide variations in banding profiles among species were observed with nearly every primer used. The results showed that foxtail millet and S. glauca had a considerable degree of variation. The geographic differentiation of RAPD patterns in foxtail millet accessions examined seemed to exist. The present study confirmed that foxtail millet is more closely related to green foxtail than to other species in Setaria, providing support for the origin of foxtail millet from S. viridis and the conspecificity of these two species. The results confirmed that S. germanica and S. italica are conspecific and the former can be replaced by the latter. Setaria glauca and S. sphacelata are distinct from foxtail millet, implying that elite traits found in these two species are relatively difficult to be used in foxtail millet improvement. This study demonstrates that RAPD analysis can be used in assessing intraspecific and interspecific variation in foxtail millet and its wild relatives.
- Darmency, H. & J. Pernes, 1985. Use of wild Setaria viridis (L.) Beauv. to improve triazine resistance in cultivated S. italica by hybridization. Weed Sci. 25: 175–179.
- de Wet, J.M.J., L.L. Oestry-Stidd & J.I. Cubero, 1979. Origins and evolution of foxtail millet. J. Agric. Trop. Bot. Appl. 26: 54–64.
- Diao, X.M., R.H. Du & R.Q. Wang, 1996. Biological performance of Setaria glauca. Millet Crops (1): 1–3.
- Jusuf, M. & J. Pernes, 1985. Genetic variability of foxtail millet (Setaria italica P. Beauv.). Electrophoretic study of five isoenzyme systems. Theor. Appl. Genet. 71: 385–391.
- Kawase, M. & S. Sakamoto, 1984. Variation, geographical distribution and genetical analysis of esterase isozymes in foxtail millet, Setaria italica (L.) P. Beauv. Theor. Appl. Genet. 67: 529–533.
- Li, C.H., W.K. Pao & H.W. Li, 1942. Interspecific crosses in Setaria. J. Hered. 33: 351–355.
- Li, H.W., C.H. Li & W.K. Pao, 1945. Cytological and genetical studies of the interspecific cross of the cultivated foxtail millet, Setaria italica (L.) Beauv., and the green foxtail millet, S. viridis L. J. Amer. Soc. Agron. 37: 32–54.
- Li, Y. & S.Z. Wu, 1996. Traditional maintenance and multiplication of foxtail millet (Setaria italica (L.) P. Beauv.) landraces in China. Euphytica 87: 33–38.
- Li, Y., S.Z. Wu & Y.S. Cao, 1995. Cluster analysis of an international collection of foxtail millet (Setaria italica (L.) P. Beauv.). Euphytica 83: 79–85.
- Li, Y., S.Z. Wu, Y.S. Cao & X.Z. Zhang, 1996. A phenotypic diversity analysis of foxtail millet (Setaria italica (L.) P. Beauv.) landraces of Chinese origin. Genet. Resour. & Crop Evol. 43: 377– 384.
- Liang, K.G., W. Liu & Y.R. Wang,,1992. Characterization of rust resistance in foxtail millet germplasm. J. Shenyang Agric. Univ. 23: 13–18.
- Naciri, Y., H. Darmency, J. Belliard, F. Dessaint & J. Pernes, 1992. Breeding strategy in foxtail millet, Setaria italica (L. P. Beauv.), following interspecific hybridization. Euphytica 60: 97–103.
- Nguyen van, E. & J. Pernes, 1985. Genetic diversity of foxtail millet (Setaria italica). In: Jacquard P, Heim G, Antonovics J (Eds) Genetic differentiation and dispersal in plants. SpringerVerlag, Berlin, pp. 113–128.
- Prasada Rao, K.E., J.M.J. de Wet, D.E. Brink & M.H. Mengesha, 1987. Infraspecific variation and systematics of cultivated Setaria italica, foxtail millet (Poaceae). Econ. Bot. 41: 108–116.
- Rohlf, F.J., 1988. NTSYSpc numerical taxonomy and multivariate analysis system, version 1.50. Exeter Publ, Setauket, New York.
- Rominger, J.M., 1962. Taxonomy of Setaria (Gramineae) in North America. Illinois Biological Monographs No. 29. University of Illinois Press, Urbana, IL
- Sharp, P.J., M. Kreis, P.R. Shewry & M.D. Gale, 1988. Location of β-amylase sequences in wheat and its relatives. Theor. Appl. Genet. 75: 286–290.
- Wang, R.L., J.F. Wendel & J.H. Dekker, 1995a. Weedy adaptation in Setaria ssp. I. Isozyme analysis of genetic diversity and population genetic structure inSetaria viridis. Amer. J. Bot. 82: 308–317.
- Wang, R.L., J.F. Wendel & J.H. Dekker, 1995b. Weedy adaptation in Setaria ssp. II. Genetic diversity and population genetic structure in S. glauca, S. geniculata, and S. faberii (Poaceae). Amer. J. Bot. 82: 1031–1039.
- Warwick, S.I., B.K. Thompson & L.D. Black, 1987. Life history and allozyme variation in populations of the weed species Setaria faberii. Can. J. Bot. 65: 1396–1402.
- Williams, R.D. & M.M. Schreiber, 1976. Numerical and chemotaxonomy of the green foxtail complex. Weed Sci. 24: 331–335.
- Zangre, G.R. & H. Darmency, 1993. Potential for selection in the progeny of an interspecific hybrid in foxtail millet. Plant Breed. 110: 172–175.
- Intraspecific and interspecific variation in Setaria revealed by RAPD analysis
Genetic Resources and Crop Evolution
Volume 45, Issue 3 , pp 279-285
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- foxtail millet
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