Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Monoclonal antibody for a ginseng saponin
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- Fukuda, N., Tanaka, H. & Shoyama, Y. Cytotechnology (2000) 34: 197. doi:10.1023/A:1008162703957
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The ratio of hapten and bovine serum albumin in an antigenconjugate was determined by matrix-assisted laserdesorption/ionization time-of-flight mass spectrometry. Ahybridoma secreting monoclonal antibody against ginsenosideRg1 was produced by fusing sprenocytes immunized with aginsenoside Rg1-bovine serum albumin conjugate withHAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very smallcross-reaction appeared with ginsenoside Re. The full measuringrange of the assay extends from 0.3 μg ml-1 to 10 μg ml-1 ofginsenoside Rg1.