European Journal of Plant Pathology

, Volume 104, Issue 4, pp 377–382

Field resistance against potato virus Y infection using natural and genetically engineered resistance genes

  • S. Dinant
  • C. Kusiak
  • B. Cailleteau
  • J.L. Verrier
  • M.C. Chupeau
  • Y. Chupeau
  • Thi Anh Hong Le
  • R. Delon
  • J. Albouy
Article

DOI: 10.1023/A:1008079003061

Cite this article as:
Dinant, S., Kusiak, C., Cailleteau, B. et al. European Journal of Plant Pathology (1998) 104: 377. doi:10.1023/A:1008079003061

Abstract

Commercial tobacco cultivars BB16 (burley) and PBD6 (dark air cured) were transformed with the coat protein gene of lettuce mosaic potyvirus (LMV). Transgenic BB16 plants showed resistance to potato virus Y (PVY) infection, against the necrotic strain PVY-N Versailles, as well as the resistance breaking necrotic strain PVY-N 107. Transgenic PBD6, which carries the recessive ‘va’ gene conferring resistance to PVY, was also protected against PVY-N 107. In the progenies of most BB16 and PBD6 transformants, 45% to 100% of the inoculated plants were immune to PVY infection. The rest of the plants were tolerant, with atypical attenuated non necrotic symptoms and reduced virus accumulation. No recovery was observed in the tolerant plants, which stably expressed detectable level of LMV coat protein. This suggested a protein-mediated mechanism of heterologous protection.

Nicotiana tabacum coat protein mediated protection 

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • S. Dinant
    • 1
    • 2
  • C. Kusiak
    • 1
  • B. Cailleteau
    • 3
  • J.L. Verrier
    • 3
  • M.C. Chupeau
    • 2
  • Y. Chupeau
    • 2
  • Thi Anh Hong Le
    • 1
  • R. Delon
    • 3
  • J. Albouy
    • 1
  1. 1.Station de Pathologie VégétaleINRAFrance
  2. 2.Laboratoire de Biologie CellulaireVersailles CxFrance
  3. 3.SEITA, Institut du TabacBergeracFrance