Cytotechnology

, Volume 29, Issue 2, pp 115–120

Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization

Article

DOI: 10.1023/A:1008068718392

Cite this article as:
Tanaka, H., Fukuda, N. & Shoyama, Y. Cytotechnology (1999) 29: 115. doi:10.1023/A:1008068718392

Abstract

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Rc and ginsenoside Rd. The full measuring range of the assay extends from 20 ngml-1 to 400 ngml-1 of ginsenoside Rb1.

ginsenoside-Rb1ELISAmonoclonal antibodyqualitative analysismass spectrometry

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Hiroyuki Tanaka
    • 1
  • Noriko Fukuda
    • 1
  • Yukihiro Shoyama
    • 1
  1. 1.Department of Pharmacognosy, Faculty of Pharmaceutical SciencesKyushu UniversityHigashi-ku, FukuokaJapan