Journal of Applied Phycology

, Volume 10, Issue 6, pp 569–572

Flow cytometric analysis of nocodazole-induced cell-cycle arrest in the pennate diatom Phaeodactylum tricornutum Bohlin

  • Christine K.F. Ng
  • Connie M.C. Lam
  • Patrick K.K. Yeung
  • J.T.Y. Wong
Article

DOI: 10.1023/A:1008039500919

Cite this article as:
Ng, C.K., Lam, C.M., Yeung, P.K. et al. Journal of Applied Phycology (1998) 10: 569. doi:10.1023/A:1008039500919

Abstract

The microtubule inhibitor, nocodazole (2.5 mg L-1), can arrest the cell cycle of the pennate diatom Phaeodactylum tricornutum Bohlin at G2 + M phase. Flow cytometric analysis of cells treated with nocodazole suggest that the proportion of cells at G2 + M phase can accumulate to over 95%. Even after a 48-h treatment with nocodazole (2.5 mg L-1), the cells can still exit mitosis, suggesting that the cell-cycle arrest is reversible.

flow cytometrydiatomPhaeodactylum tricornutumcell cyclenocodazole

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Christine K.F. Ng
    • 1
  • Connie M.C. Lam
    • 1
  • Patrick K.K. Yeung
    • 1
  • J.T.Y. Wong
    • 1
  1. 1.Biology DepartmentHong Kong University of Science and TechnologyKowloon, Hong KongChina