Preparation of monoclonal antibody against crocin and its characterization
- Cite this article as:
- Xuan, L., Tanaka, H., Xu, Y. et al. Cytotechnology (1999) 29: 65. doi:10.1023/A:1007993615489
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Three crocin-carrier protein conjugates were synthesized and their hapten numbers were determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Three monoclonal antibodies against crocin were produced by hybridomas fused with the splenocytes immunized with crocin hemisuccinate-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. They were identified as IgG2a and IgG2b possessing λ light chain, respectively. Their wide reactivities against crocetin glycosides were discussed.