Cytotechnology

, Volume 29, Issue 1, pp 65–70

Preparation of monoclonal antibody against crocin and its characterization

  • Lijiang Xuan
  • Hiroyuki Tanaka
  • Yaming Xu
  • Yukihiro Shoyama
Article

DOI: 10.1023/A:1007993615489

Cite this article as:
Xuan, L., Tanaka, H., Xu, Y. et al. Cytotechnology (1999) 29: 65. doi:10.1023/A:1007993615489

Abstract

Three crocin-carrier protein conjugates were synthesized and their hapten numbers were determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Three monoclonal antibodies against crocin were produced by hybridomas fused with the splenocytes immunized with crocin hemisuccinate-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. They were identified as IgG2a and IgG2b possessing λ light chain, respectively. Their wide reactivities against crocetin glycosides were discussed.

crocetin glycosides crocin Crocus sativus ELISA MALDI-TOF-MS monoclonal antibody 

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Lijiang Xuan
    • 1
  • Hiroyuki Tanaka
    • 1
  • Yaming Xu
    • 2
  • Yukihiro Shoyama
    • 1
  1. 1.Department of Pharmacognosy, Faculty of Pharmaceutical SciencesKyushu UniversityHigashiku, FukuokaJapan
  2. 2.Shanghai Institute of Materia Medica, Chinese Academy of SciencesShanghaiChina