Virus Genes

, Volume 14, Issue 2, pp 105–109

Identification and Characterization of the Feline Herpesvirus Type 1 Glycoprotein C Gene

  • Ken Maeda
  • Kentaro Fujita
  • Naoaki Yokoyama
  • Takeshi Mikami
Article

DOI: 10.1023/A:1007961100023

Cite this article as:
Maeda, K., Fujita, K., Yokoyama, N. et al. Virus Genes (1997) 14: 105. doi:10.1023/A:1007961100023

Abstract

The feline herpesvirus type 1 (FHV-1) gene encoding glycoprotein C (gC) has been sequenced and identified based on its genomic location and comparative analysis to other alphaherpesvirus gCs, and the expressed gC protein was also identified by using specific monoclonal antibodies. The FHV-1 gC gene was located within a 7.0 kbp EcoRI fragment, and was 1602 bp in length. The amino acid sequence deduced from the nucleotide sequence was predicted to encode a membrane glycoprotein containing a characteristic N-terminal hydrophobic signal sequence, nine potentialN -linked glycosylation sites, and C-terminal transmembrane and cytoplasmic domains. The FHV-1 gC was expressed in COS-7 cells. When flowcytometric analysis was carried out, the gC expressed in COS-7 cells reacted with a panel of monoclonal antibodies against gp113. By immunoprecipitation analysis, the gC expressed in COS-7 cells possessed molecular masses of 125–;150 kilodalto n, and was similar in size to that in FHV-1-infected CRFK cells.

feline herpesvirus type l glycoprotein C gp113 

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Ken Maeda
    • 1
  • Kentaro Fujita
    • 1
  • Naoaki Yokoyama
    • 1
  • Takeshi Mikami
    • 1
  1. 1.Department of Veterinary Microbiology, Faculty of AgricultureThe University of TokyoTokyoJapan