Journal of Applied Phycology

, 9:29

A comparison of screening methods for antioxidant activity in seaweeds

  • R. Matsukawa
  • Z. Dubinsky
  • E. Kishimoto
  • K. Masaki
  • Y. Masuda
  • T. Takeuchi
  • M. Chihara
  • Y. Yamamoto
  • E. Niki
  • I. Karube
Article

DOI: 10.1023/A:1007935218120

Cite this article as:
Matsukawa, R., Dubinsky, Z., Kishimoto, E. et al. Journal of Applied Phycology (1997) 9: 29. doi:10.1023/A:1007935218120

Abstract

The inhibition of lipid peroxidation and radical scavenging effects were studied to evaluate the antioxidant activity for extracts of 17 species of seaweed. The antioxidant effect was evaluated by determination of lipoxygenase activity and by α, α-diphenyl-β-picrylhydrazyl (DPPH) decolorization. Lipoxygenase activity was depressed in the presence of aqueous and ethanol extracts of 4 algal species; Sargassum species had the highest antioxidant activity of all the species examined. The ethanol extracts of one Sargassum species showed competitive inhibition with the substrate. The same species also showed radical scavenging activity in the DPPH decolorization test. Comparison of these results shows no relationship between enzyme inhibition and radical scavenging activity.

antioxidant activityscreeninglipoxygenase inhibitionradical scavenging activityseaweed

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • R. Matsukawa
    • 1
  • Z. Dubinsky
    • 2
  • E. Kishimoto
    • 3
  • K. Masaki
    • 4
  • Y. Masuda
    • 3
  • T. Takeuchi
    • 5
  • M. Chihara
    • 1
    • 6
  • Y. Yamamoto
    • 1
  • E. Niki
    • 1
  • I. Karube
    • 1
  1. 1.Research Center for Advanced Science and TechnologyThe University of TokyoMeguro-ku, TokyoJapan
  2. 2.Department of Life ScienceBar-Ilan UniversityRamat-GanIsrael
  3. 3.Faculty of EngineeringThe Science University of TokyoShinjuku-ku, TokyoJapan
  4. 4.Toyo Susan Kaisha LtdTokyoJapan
  5. 5.Faculty of Information ScienceHiroshima City UniversityNumata-cho, Asaminami-ku, HiroshimaJapan
  6. 6.Japanese Red Cross College of NursingShibuya-ku, TokyoJapan