Cytotechnology

, Volume 26, Issue 2, pp 153–164

Effect of culture temperature on a recombinant CHO cell line producing a C-terminal α-amidating enzyme

  • Kazuaki Furukawa
  • Kazuhiro Ohsuye
Article

DOI: 10.1023/A:1007934216507

Cite this article as:
Furukawa, K. & Ohsuye, K. Cytotechnology (1998) 26: 153. doi:10.1023/A:1007934216507

Abstract

In order to seek an efficient method for producing a recombinant protein by using animal cell culture, we investigated various effects of the culture temperature on a recombinant CHO cell line (3µ-1S), producing a C-terminal α-amidating enzyme (799BglIIα-AE) originating from Xenopus laevis. The results revealed that a low culture temperature (below 37 °C) led to the following phenomena: [1] inhibited cell growth, [2] enhanced cellular productivity of the recombinant protein, [3] maintained high cell viability, [4] suppressed medium consumption, and [5] suppressed release of impurities from the cells. These findings indicate that a quite simple method, the culture at low temperature, will contribute to the total improvement of the industrial process for the production of the recombinant protein, 799BglIIα-AE.

animal cell culturecellular productivityC-terminal α-amidating enzymeculture temperaturerecombinant CHO cell line

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Kazuaki Furukawa
    • 1
  • Kazuhiro Ohsuye
    • 1
  1. 1.Suntory Institute for Medicinal Research and DevelopmentAkaiwa, Chiyoda-machi, Ohra-gun, GunmaJapan