Effect of culture temperature on a recombinant CHO cell line producing a C-terminal α-amidating enzyme
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- Furukawa, K. & Ohsuye, K. Cytotechnology (1998) 26: 153. doi:10.1023/A:1007934216507
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In order to seek an efficient method for producing a recombinant protein by using animal cell culture, we investigated various effects of the culture temperature on a recombinant CHO cell line (3µ-1S), producing a C-terminal α-amidating enzyme (799BglIIα-AE) originating from Xenopus laevis. The results revealed that a low culture temperature (below 37 °C) led to the following phenomena:  inhibited cell growth,  enhanced cellular productivity of the recombinant protein,  maintained high cell viability,  suppressed medium consumption, and  suppressed release of impurities from the cells. These findings indicate that a quite simple method, the culture at low temperature, will contribute to the total improvement of the industrial process for the production of the recombinant protein, 799BglIIα-AE.