Journal of Applied Phycology

, Volume 9, Issue 3, pp 287–293

Expressed sequence tags (ESTs) from the marine red alga Gracilaria gracilis

  • Arturo O. Lluisma
  • Mark A. Ragan
Article

DOI: 10.1023/A:1007920607900

Cite this article as:
Lluisma, A.O. & Ragan, M.A. Journal of Applied Phycology (1997) 9: 287. doi:10.1023/A:1007920607900

Abstract

Expressed sequence tags (ESTs) are partial sequences of cDNAs, and can be used to characterize gene expression in organisms or tissues. We have constructed a 200-sequence EST database from vegetative thalli of Gracilaria gracilis, the first ESTs reported from any alga. This database contains recognizable ESTs corresponding to genes of carbohydrate metabolism (seven), amino acid metabolism (three), photosynthesis (five), nucleic acid synthesis, repair and processing (three), protein synthesis (14), protein degradation (six), cellular maintenance and stress response (three), other identifiable protein-coding genes (13) and 146 sequences for which significant matches were not found in existing sequence databases. We have already used this EST database to recover genes of carbohydrate biosynthesis from G. gracilis.

ESTexpressed sequence tagGracilariacarbohydrate biosynthesis

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Arturo O. Lluisma
    • 1
    • 2
  • Mark A. Ragan
    • 1
    • 4
  1. 1.Institute for Marine BiosciencesNational Research Council (Canada)HalifaxCanada
  2. 2.Department of BiologyDalhousie UniversityHalifaxCanada
  3. 3.Marine Science Institute, University of the PhilippinesQuezon CityPhilippines
  4. 4.Program in Evolutionary BiologyCanadian Institute for Advanced ResearchCanada