Microbial Diversity in Nankai Trough Sediments at a Depth of 3,843 m
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
Dense populations of bivalves, primarily Calyptogena sp., were observed at cold seeps of the Nankai Trough. Bacterial input to the sediment was estimated through determination of phospholipid ester-linked fatty acid (PLFA) and DNA profiles. Results indicated a bacterial biomass of 109 cells (g dry wt)-1 while individual fatty acid profiles revealed a predominance of monounsaturated fatty acids, mainly 18:1 isomers. The presence of these fatty acids can be interpreted to reflect a response to low temperature and a predominance of psychrophilic bacteria. DNA fragments encoding bacterial ribosomal RNA small-subunit sequences (16S rDNA) were amplified by the polymerase chain reaction method using DNA extracted directly from the sediment samples. From the sequencing results, at least 19 kinds of bacterial 16S rDNAs related to mostly the Proteobacteria and a few gram-positive bacteria were identified. These results suggest that the bacterial community in the Nankai Trough sediments consists of mainly bacteria belonging to the Proteobacteria γ, ε, and δ subdivisions. Bacteria belonging to the ε and δ subdivisions, which are known to include epibiont and sulfate reducing bacteria, respectively, were mostly detected in the sediment obtained from inside the area of the Calyptogena community, and the δ-Proteobacteria may function to supply reduced sulfur to bacterial endosymbionts of Calyptogena.
- Ashi, J. (1997): Distribution of cold seepage at the fault scarp of the eastern Nankai accretionary prism. JAMSTEC J. Deep Sea Res., 13, 495–501.
- Balkwill, D. L., F. R. Leach, J. T. Wilson, J. F. McNabb and D. C. White (1988): Equivalence of microbial biomass measures based on membrane lipid and cell wall components, adenosine triphosphate, and direct counts in subsurface sediments. Microb. Ecol., 16, 73–84.
- Ben-Mlih, F., J.-C. Marty and A. Fiala-Medioni (1992): Fatty acid composition in deep-sea hydrothermal vent symbiotic bivalves. J. Lipid Res., 33, 1797–1806.
- Bligh, E. G. and W. J. Dyer (1959): A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol., 37, 911–917.
- Boon, J. J., J. W. De Leeuw, G. J. Van Der Hoek and J. H. Vosjan (1977): Significance and taxonomic value of iso and anteiso monoenoïic fatty acids and branched beta hydroxy fatty acids in Desulfovibrio desulfuricans. J. Bacteriol., 129, 1183–1191.
- Bowman, J. P., J. Cavanagh, J. J. Austin and K. Sanderson (1996): Novel Psychrobacter species from Antarctic ornithogenic soils. Int. J. Syst. Bacteriol., 46, 841–848.
- DeLong, E. F. (1992): Archaea in coastal marine environments. Proc. Natl. Acad. Sci. USA, 89, 5685–5689.
- Distel, D. L., D. J. Lane, G. J. Olsen, S. J. Giovannoni, B. Pace, N. R. Pace, D. A. Stahl and H. Felbeck (1988): Sulfur-oxidizing bacterial endosymbionts: analysis of phylogeny and specificity by 16S rRNA sequences. J. Bacteriol., 170, 2506–2510.
- Dowling, N. J. E., J. G. Guezennec and D. C. White (1988): Methods for insight into mechanisms of microbially influenced metal corrosion. p. 404–410. In Biodeterioration, ed. by D. R. Hougton, R. T. Smith and H. O. U. Eggins, Elsevier Applied Science Publ., London.
- Gamo, T., J. Ishibashi, K. Shitashima, T. Nakatsuka, U. Tsunogai, T. Masuzawa, H. Sakai and K. Mitsuzawa (1994): Chemical characteristics of cold seepage at the eastern Nankai Trough accretionary prism (KAIKO-TOKAI Program): a preliminary report of the dive 113 of “Shinkai 6500”. JAMSTEC J. Deep Sea Res., 10, 343–352.
- Gill, C. O. (1975): Effect of growth temperature on the lipids of Pseudomonas fluorescens. J. Gen. Microbiol., 89, 293–298.
- Guckert, J. B., C. P. Anthworth, P. D. Nichols and D. C. White (1985): Phospholipid ester-linked fatty acid profiles as reproducible assays for changes in prokaryotic community structure of estuarine sediments. FEMS Microbiol. Ecol., 31, 147–158.
- Guezennec, J. G. (1991): Influence of cathodic protection of mild steel on the growth of sulphate-reducing bacteria at 35°C in marine sediments. Biofouling, 3, 339–348.
- Haddad, A., F. Camacho, P. Durand and S. C. Cary (1995): Phylogenetic characterization of the epibiotic bacteria associated with the hydrothermal vent polychaete Alvinella pompejana. Appl. Environ. Microbiol., 61, 1679–1687.
- Henderson, R. J., R. M. Millar, J. R. Sargent and J. P. Jostensen (1993): Trans-monoenoic and polyunsaturated fatty acids in phospholipids of a Vibrio species of bacterium in relation to growth conditions. Lipids, 28, 389–396.
- Kato, C., L. Li, J. Tamaoka and K. Horikoshi (1997): Molecular analyses of the sediment of the 11000m deep Mariana Trench. Extremophiles, 1, 117–123.
- Keweloh, H. and H. J. Heipieper (1996): Trans unsaturated fatty acids in bacteria. Lipids, 31, 129–137.
- Le Chevalier, M. P. (1977): Lipids in bacterial taxonomy—A taxonomist's view. Crit. Rev. Microbiol., 7, 109–210.
- Le Pichon, X., T. Liyama, J. Boulegue, J. Charvet, M. Faure, K. Kano, S. Lallemant, H. Okada, C. Rangin, A. Taira, T. Urabe and S. Uyeda (1987): Nankai Trough and Zenisu Ridge: A deep-sea submersible survey. Earth Planet Sci. Lett., 83, 285–299.
- Le Pichon, X. and Kaiko-Nankai Scientific Crew (1992): Fluid venting activity within the eastern Nankai Trough accretionary wedge: A summary of the 1989 Kaiko-Nankai results. Earth Planet Sci. Lett., 109, 303–318.
- Marr, A. G. and J. L. Ingraham (1962): Effect of temperature on the composition of fatty acids in Escherichia coli. J. Bacteriol., 84, 1260–1267.
- Masuzawa, T., N. Handa, H. Kitagawa and M. Kusakabe (1992): Sulfate reduction using methane in sediments beneath a bathyal “cold seep” giant clam community off Hatsushima Island, SagamiBay, Japan. Earth Planet Sci. Lett., 110, 39–50.
- Naganuma, T., C. Kato, H. Hirayama, N. Moriyama, J. Hashimoto and K. Horikoshi (1997): Intracellular occurrence of ɛ-Proteobacterial 16S rDNA sequences in the Vestimentiferan trophosome. J. Oceanogr, 53, 193–197.
- Saitou, N. and M. Nei (1987): The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol., 4, 406–425.
- Sambrook, J., E. F. Fritsch and T. Maniatis (1989): Molecular cloning: a laboratory manual. Cold Spring Harbor, New York.
- Suzuki, M. T., M. S. Rappe, Z. W. Haimberger, H. Winfield, N. Adair, J. Strobel and S. J. Giovannoni (1997): Bacterial diversity among small-subunit rRNA gene clones and cellular isolates from the same seawater sample. Appl. Environ. Microbiol., 63, 983–989.
- Vincent, P., P. Pignet, F. Talmont, L. Bozzi, B. Fournet, J. Guezennec, C. Jeanthon and D. Prieur (1994): Production and characterization of an exopolysaccharide excreted by a deepsea hydrothermal vent bacterium isolated from the polychaete Alvinella pompejana. Appl. Environ. Microbiol., 60, 4134–4141.
- Ward, D. M., M. M. Bateson, R. Weller and A. L. Ruff-Roberts (1992): Ribosomal RNA analysis for microorganisms as they occur in nature. Adv. Microbiol. Ecol., 12, 219–286.
- White, D. C. (1988): Validation of quantitative analysis for microbial biomass, community structure, and metabolic activity. Adv Limnol., 31, 1–18.
- White, D. C., W. M. Davis, J. S. Nickels, J. D. King and R. J. Bobbie (1979): Determination of the sedimentary microbial biomass by extractable lipid phosphate. Oceologia, 40, 51–62.
- Microbial Diversity in Nankai Trough Sediments at a Depth of 3,843 m
Journal of Oceanography
Volume 55, Issue 5 , pp 635-642
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- microbial diversity
- cold seep
- Nankai Trough
- 16S ribosomal RNA gene
- Author Affiliations
- 1. Japan Marine Science and Technology Center (JAMSTEC), The DEEPSTAR Group, 2-15 Natsushima-cho, Yokosuka, 237-0061, Japan
- 2. Dept DRV/VP/BMH, IFREMER, BP 70, 29280, Plouzane, France
- 3. Division of Marine Research, CSIRO, GPO Box 1358, Hobart, TAS, 7001, Australia
- 4. ENS, Lab, de Géologie 24, Rue L'Homond, 75231, Paris Cedex 05, France