Fish Physiology and Biochemistry

, Volume 16, Issue 6, pp 479–485

Dietary spermine supplementation induces intestinal maturation in sea bass (Dicentrarchus labrax) larvae

Authors

  • A. Péres
    • Unité Mixte de Nutrition des Poissons IFREMER-INRAIFREMER Centre de Brest
  • C.L. Cahu
    • Unité Mixte de Nutrition des Poissons IFREMER-INRAIFREMER Centre de Brest
  • J.L. Zambonino Infante
    • Unité Mixte de Nutrition des Poissons IFREMER-INRAIFREMER Centre de Brest
Article

DOI: 10.1023/A:1007786128254

Cite this article as:
Péres, A., Cahu, C. & Zambonino Infante, J. Fish Physiology and Biochemistry (1997) 16: 479. doi:10.1023/A:1007786128254

Abstract

Sea bass (Dicentrarchus labrax) larvae were fed microparticulated compound diet containing 0 (FP0), 0.10 (FP10) and 0.33% (FP33) of a polyamine, spermine, from day 20 to day 38. LP group was fed live prey. This group exhibited the highest growth and survival. The addition of spermine did not lead to growth enhancement. A 33% survival improvement was obtained in FP33 group compared to FP0 group. The spermine addition affected the activity of pancreatic enzymes, trypsin, chymotrypsin and amylase, during larvae development. This non specific effect suggested that the action of spermine would be mediated by hormones. In the intestine, the FP33 group exhibited from day 31 higher activities of brush border membrane enzymes (leucine aminopeptidase and alkaline phosphatase) and lower level in a cytosolic enzyme (leucine-alanine peptidase) compared to FP10 and FP0 group. The diet containing the highest spermine level induced an enzymatic profile similar to that obtained in LP group and characteristic of a mature enterocyte. The initiation of enterocyte maturation at a proper development stage was associated to the survival improvement observed in FP33 group.

polyaminesperminesea basslarvaeintestinal maturationpancreatic enzymesintestinal enzymes

Copyright information

© Kluwer Academic Publishers 1997