European Journal of Epidemiology

, Volume 15, Issue 7, pp 665–669

Genetic diversity of Borrelia burgdorferi sensu lato isolates obtained from Ixodes ricinus ticks collected in Slovakia

  • Lise Gern
  • Chang Min Hu
  • Elena Kocianova
  • Vanda Vyrostekova
  • J. Rehacek
Article

DOI: 10.1023/A:1007660430664

Cite this article as:
Gern, L., Hu, C.M., Kocianova, E. et al. Eur J Epidemiol (1999) 15: 665. doi:10.1023/A:1007660430664

Abstract

In Europe, Borrelia burgdorferi sensu lato is diverse, including B.burgdorferi s.s., B.garinii, B.afzelii, B.valaisiana and B.lusitaniae. In this study, we focused on the distribution of the different B.burgdorferi species among Ixodes ricinus adult ticks collected in an endemic area within Slovakia. We compared results of prevalence of B.burgdorferi infection in ticks obtained by immunofluorescence (IF) and by isolation. Isolates were characterized by restriction fragment length polymorphism (RFLP) of the rrf-rrl intergenic spacer genes using MseI. Using immunofluorescence we observed that 56/114 (49%) ticks were infected by B.burgdorferi s.l. Males were found to be more often infected (32/57, 56%) than females (24/57, 42%) but the difference was not significant (p = 0.1895). From the same 114 ticks a total of 37 isolates were obtained: 19 from males (33%) and 18 from females (32%). The RFLP identification revealed 25 B.afzelii (68%), 5 B.garinii (14%), 5 B.valaisiana (14%) and 2 B.lusitaniae (5%). The infection in ticks was more often detected by IF than by isolation (p = 0.0153) and isolation success was higher when the infection degree in ticks was high (p = 0.0397). The infection prevalence observed in this area is among the highest observed in Europe.

Borrelia burgdorferi s.l. Immunofluorescence Isolation Ixodes ricinus 
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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Lise Gern
    • 1
  • Chang Min Hu
    • 1
  • Elena Kocianova
    • 2
  • Vanda Vyrostekova
    • 3
  • J. Rehacek
    • 2
  1. 1.Institute of ZoologyUniversity of NeuchâtelSwitzerland
  2. 2.Institute of VirologySlovak Academy of SciencesBratislavaSlovak Republic
  3. 3.Institute of Epidemiology, Medical FacultyComenius UniversityBratislavaSlovak Republic

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